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Human cytomegalovirus reprograms hematopoietic progenitor cells into immunosuppressive monocyte to achieve latency

ABSTRACT: HCMV NR-1 infection reprograms human CD34+ HPCs into long-life, NO-producing, immune suppressive Mo-MDSC-like cells at the late stage of infection We used microarray to detail the gene expression of human CD34+ HPCs and long-life, NO-producing, immune suppressive Mo-MDSC-like cells at the late stage of infection

ORGANISM(S): Homo sapiens

PROVIDER: GSE106879 | GEO | 2017/11/15

SECONDARY ACCESSION(S): PRJNA418309

REPOSITORIES: GEO

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Comparative quantitative proteomic analysis of HCMV infected cells highlights pUL138 as a multiple functional protein

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Monocytic myeloid-derived suppressor cells accumulate in the lungs of influenza A virus-infected mice

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Project description:Study of human monocytic Myeloid-Derived Suppressor cells Mo-MDSC (CD14+ HLA-DRneg/low) has been hampered by the lack of positive cell-surface markers. In order to identify positive markers for Mo-MDSC, we performed microarray analysis comparing Mo-MDSC cells from healthy subjects versus CD14+ HLA-DRhigh monocytes. We have identified the surface ectoenzyme Vanin-2(VNN2) protein as a novel biomarker highly-enriched in healthy subjects Mo-MDSC. Indeed, healthy subjects Mo-MDSC cells expressed 68% VNN2, whereas only 9% VNN2 expression was observed on CD14+ HLA-DRhigh cells (n=4 p<0.01). The top 10 percent positive VNN2 monocytes expressed CD33 and CD11b while being negative for HLA-DR, CD3, CD15, CD19 and CD56, consistent with a Mo-MDSC phenotype. CD14+VNN2high monocytes were able to inhibit CD8 T cell proliferation comparably to traditional Mo-MDSC at 51% and 48% respectively. However, VNN2 expression on CD14+ monocytes from glioma patients was inversely correlated to their grade. CD14+VNN2high monocytes thus appear to mark a monocytic population similar to Mo-MDSC only in healthy subjects, which may be useful for tumor diagnoses. Myeloid-Derived Suppressor Cells (MDSC) are identified by upregulated expression of the cell surface ectoenzyme Vanin-2 in healthy subjects

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