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RNA sequencing of human periodontal ligament stem cells (PDLSCs) with Illumina HiSeq2000

ABSTRACT: To study the molecular mechanisms underlying PDLSC osteogenic differentiation, we developed RNA sequencing with Illumina HiSeq2000 to comprehensively identify RNA expression profiles in normal PDLSCs, osteogenic inductive PDLSCs and mechanical force-induced PDLSCs. Plenty of RNAs were found to be differentially expressed by RNA sequencing. Furthermore, the potential functions of these RNAs were investigated using bioinformatic analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE106887 | GEO | 2018/03/09

REPOSITORIES: GEO

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The long noncoding RNA AC018926.2 plays a crucial role in palmitic acid exposure-compromised osteogenic potential of periodontal ligament stem cells

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Hyaluronic Acid Induction Promotes the Differentiation of Human Neural Crest-like Cells into Periodontal Ligament Stem-like Cells

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2024-07-31 | GSE264552 | GEO

microRNA expression of human periodontal ligament derived stem cell line exposed to 1 microMolar (uM) (-)-nicotine liquid or 1 uM nicotine electronic cigarette vapor extract against untreated control

Project description:Nicotine and cigarette smoking have been previously shown to inhibit the differentiation potential and miRNA expression of human periodontal ligament derived stem cells (PDLSCs). Electronic cigarette (EC) vapor also contains nicotine, as well as other non-nicotine compounds, and, like cigarette smoking, EC vaping results in similar routes of toxic exposure for PDLSCs. The effect of exposure on PDLSC miRNA expression is unknown. To determine these effects, cultured PDLSCs were exposed to media supplemented with 1 uM nicotine EC vapor extract for 72h, during which media was changed every 24h. To produce vapor extract, an EC filled with 36 mg/ml nicotine, 50%/50% (w/v) PG/VG, non-flavored e-liquid (American E-liquid) was vaped using an automated vaping robot programmed with experienced e-cigarette user patterns (i.e. puff volume: 70 ml, duration: 3s, frequency: 20s) and resulting vapor was extracted via a liquid impinger. 1 uM of pure (-)-nicotine liquid (Sigma Aldrich) was used as a positive control. Total miRNA was collected with the mirVana miRNA isolation kit. miRNA concentration and quality was measured by spectrophotometry and expression was analyzed with Affymetrix GeneChip miRNA 4.0 Arrays. Results were normalized against human-positive and negative chip controls using Robust Multi-Array Average Normalization.

2019-09-30 | E-MTAB-7893 | biostudies-arrayexpress

RNA sequencing of human periodontal ligament stem cells (PDLSCs) with Illumina HiSeq2000

Project description:RNA sequencing of human periodontal ligament stem cells (PDLSCs) with Illumina HiSeq2000

| PRJNA418326 | ENA

Gene expression profiling of KSHV-infected periodontal ligament cells

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2017-06-01 | GSE92341 | GEO

Proteomic and Phosphoproteomic Analyses of the Molecular Mechanisms of Periodontal Ligament Stem Cell Osteogenic Differentiation

Project description:Periodontal ligament stem cells (PDLSCs) are optimal seed cells for periodontal regeneration; thus, exploration of the mechanisms regulating PDLSCs osteogenic differentiation is critical. Here, tandem mass tag quantitative proteomics and phosphoproteomic analyses of human PDLSCs were carried out after osteogenic differentiation for 14 days. Proteomic analyses identified 314 differentially expressed proteins (DEPs) that were predicted to be mostly extracellular. Gene ontology and pathway enrichment analyses revealed that the mineral absorption signaling pathway was significantly involved. For phosphoproteomics, un-normalized data identified 1016 differentially expressed phosphorylation sites (DEPSs) corresponded to 380 DEPs, whereas in normalized data, 3030 DEPSs were identified, corresponding to 358 DEPs. Overlapping of these data identified 16 phosphorylated DEPs, among which tetratricopeptide repeat protein 17, stanniocalcin-1, and extracellular superoxide dismutase [Cu-Zn] were significantly related. Kyoto

2022-02-25 | PXD031905 |

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