Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice

ABSTRACT: Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Keywords: knockout effect

ORGANISM(S): Mus musculus

PROVIDER: GSE10740 | GEO | 2008/03/06

SECONDARY ACCESSION(S): PRJNA107513

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of mouse colon from wild-type and Slc9a3 (NHE3)-deficient animals

Project description:Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Experiment Overall Design: Whole colon was dissected from 6-8 week old NHE3-deficient mice and their wild-type littermates and total RNA isolated for microarray analysis using Affymetrix murine MOE430 2.0 arrays

2008-04-06 | E-GEOD-10740 | biostudies-arrayexpress

Coordinated assembly of NHE3, IRBIT, NHERF1, and ezrin ameliorates fluid malabsorption in diabetes

Project description:Clinical evidence supports the occurrence of intermittent diarrhea in many type 1 diabetes mellitus (T1DM) patients. Others and we found that net fluid absorption rate is dramatically lower in the ileum of T1DM murine models. However, the identity of molecules that contribute to fluid malabsorption in the gut remains unknown. Considering the importance of ion transporters and channels for intestinal fluid absorption, we reasoned that their expression level at the luminal membrane is altered under diabetic conditions. To this end, we analyzed the brush border membrane vesicles (BBMVs) from the ileum of control and DM mice by proteomic analysis. The expression levels of Cl-/HCO3- exchanger SLC26A3/DRA and cystic fibrosis transmembrane conductance regulator (CFTR) were not significantly different between control and DM mice. Cl-/HCO3- exchanger Slc26a6/PAT1 and Na+/H+ exchanger 3 (NHE3) were not detected. Interestingly, cytoskeleton scaffold proteins that regulate NHE3, including NHERF1-3 and ezrin, were all significantly lower in diabetic animals.

2016-04-27 | PXD001859 | Pride

Effects of NHE3 knockdown on the transcriptome of human colonic adenocarcinoma cells SK-CO15

Project description:NHE3 expression is downregulated in human colerectal cancer. This study evaluates the influence of of ca. 70% decrease in NHE3 expression on the transcriptomic profile of human colonic adenocarcinoma cells SK-CO15

2021-09-20 | GSE184326 | GEO

Global gene expression profile of small intestine from wild-type and NHE3-knockout mice.

Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NHE3-knockout small intestine. Keywords: gene expression comparison

2008-08-08 | GSE8971 | GEO

Global gene expression profile of small intestine from wild-type and NHE3/CFTR double knockout mice.

Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NHE3/CFTR double knockout small intestine. Keywords: gene expression comparison

2008-08-08 | GSE9009 | GEO

Effects of NHE3 knockdown on the transcriptome of human colonic adenocarcinoma cells SK-CO15

Project description:Effects of NHE3 knockdown on the transcriptome of human colonic adenocarcinoma cells SK-CO15

| PRJNA764079 | ENA

Disruption of Na+/H+ exchanger regulatory factor 2 scaffold suppresses colon cancer proliferation

Project description:A key function of Na+/H+ exchanger regulatory factor 2 (NHERF2) is spatial organization of signaling proteins to facilitate signal transduction. The role of NHERF2 in cancer progress is not well understood. This study determines how loss of NHERF2 alter colon cancer progress.

2016-05-05 | GSE67931 | GEO

Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [intestinal epithelial cells]

Project description:G. lamblia is a fecal-oral transmitted human enteropathogenic protozoan with extremely high incidence in endemic areas in Africa and Asia particularly in the infant population. It homes to the proximal small intestine and induced diarrhea and malabsoption. Here we established a novel murine G. lamblia infection model and analyzed the tissue response and downstream microbial and metabolic effects in the host.

2020-06-01 | GSE113667 | GEO

Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [small intestinal tissue]

2020-06-01 | GSE113666 | GEO

Influence of G. lamblia infection on the transcriptional profile in intestinal and liver tissue [liver]

2020-06-01 | GSE113679 | GEO