Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global gene expression profile of small intestine from wild-type and NHE3/CFTR double knockout mice.

ABSTRACT: The objective of this study was to identify changes in gene expression levels between wild-type and NHE3/CFTR double knockout small intestine. Keywords: gene expression comparison

ORGANISM(S): Mus musculus

PROVIDER: GSE9009 | GEO | 2008/08/08

SECONDARY ACCESSION(S): PRJNA102493

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Global gene expression profile of small intestine from wild-type and NHE3-knockout mice.

Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NHE3-knockout small intestine. Keywords: gene expression comparison

2008-08-08 | GSE8971 | GEO

Global gene expression profile of small intestine from wild-type and CFTR-knockout mice.

2008-08-08 | GSE8763 | GEO

Global gene expression profile of small intestine from wild-type and CFTR-knockout mice.

Project description:The objective of this study was to identify changes in gene expression levels between wild-type and CFTR-knockout small intestine. CFTR-knockout mice (provided by Dr. Lane Clarke of the University of Missouri) were maintained on colyte. Keywords: gene expression comparison Four wild-type and four CFTR-knockout small intestinal RNA samples were compared. To facilitate statistical analysis and reduce affects of Cy3 and Cy5 labeling, comparison of two WT and two KO were repeated with a dye flip.

2010-05-25 | E-GEOD-8763 | biostudies-arrayexpress

Coordinated assembly of NHE3, IRBIT, NHERF1, and ezrin ameliorates fluid malabsorption in diabetes

Project description:Clinical evidence supports the occurrence of intermittent diarrhea in many type 1 diabetes mellitus (T1DM) patients. Others and we found that net fluid absorption rate is dramatically lower in the ileum of T1DM murine models. However, the identity of molecules that contribute to fluid malabsorption in the gut remains unknown. Considering the importance of ion transporters and channels for intestinal fluid absorption, we reasoned that their expression level at the luminal membrane is altered under diabetic conditions. To this end, we analyzed the brush border membrane vesicles (BBMVs) from the ileum of control and DM mice by proteomic analysis. The expression levels of Cl-/HCO3- exchanger SLC26A3/DRA and cystic fibrosis transmembrane conductance regulator (CFTR) were not significantly different between control and DM mice. Cl-/HCO3- exchanger Slc26a6/PAT1 and Na+/H+ exchanger 3 (NHE3) were not detected. Interestingly, cytoskeleton scaffold proteins that regulate NHE3, including NHERF1-3 and ezrin, were all significantly lower in diabetic animals.

2016-04-27 | PXD001859 | Pride

CFTR is a tumor suppressor gene in murine and human intestinal cancer [microarray]

Project description:Analysis of the cystic fibrosis gene Cftr in the colon and small intestine of Cftr-deficient murine model. The hypothesis was loss of Cftr altered expression of genes important in intestinal homeostasis and oncogenic signaling pathways. The results identified potential roles of Cftr in up- or down-regulating major gene clusters that belong to groups of immune response, ion channel, intestinal stem cell and other growth regulators. Total RNA was isolated from the normal intestine of three Apc wildtype Cftr wildtype and three Apc Cftr-deficient mice. For the colon intestinal epithelia from the same region of the distal colon of each mouse was separated from the rest of the intestine prior to RNA isolation. Therefore RNA was obtained from only epithelial cells. For the small intestine, a section of the mid-duodenum from each mouse was sheared of villi prior to RNA isolation. Therefore RNA was obtained from whole duodenum (minus villi), containing epithelia cells but also stromal and other cells. RNA Seq was then conducted on all samples, with at least two replicates for each biological sample.

2015-12-15 | E-GEOD-75996 | biostudies-arrayexpress

Transcription profiling of mouse colon from wild-type and Slc9a3 (NHE3)-deficient animals

Project description:Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Experiment Overall Design: Whole colon was dissected from 6-8 week old NHE3-deficient mice and their wild-type littermates and total RNA isolated for microarray analysis using Affymetrix murine MOE430 2.0 arrays

2008-04-06 | E-GEOD-10740 | biostudies-arrayexpress

Global gene expression profile of small intestine from wild-type and NHE2-knockout mice.

Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NHE2-knockout small intestine.

2010-12-02 | GSE19333 | GEO

Global gene expression profile of small intestine from wild-type and NKCC1 (SLC12A2)-knockout mice.

Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NKCC1-knockout small intestine.

2010-11-17 | GSE19117 | GEO

Expression data from the colon of wild-type and Slc9a3 (NHE3)-deficient mice

2008-03-06 | GSE10740 | GEO

Effects of NHE3 knockdown on the transcriptome of human colonic adenocarcinoma cells SK-CO15

Project description:NHE3 expression is downregulated in human colerectal cancer. This study evaluates the influence of of ca. 70% decrease in NHE3 expression on the transcriptomic profile of human colonic adenocarcinoma cells SK-CO15

2021-09-20 | GSE184326 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data