Transcriptomics

Dataset Information

0

Bifidobacterium kashiwanohense expresses fucosidases necessary for fucosyllactose utilisation as an adaptation to the infant gut environment


ABSTRACT: Bifidobacteria dominate the composition of the neonatal gut microbiota in the first number of weeks following birth. A number of species in particular are found with a significantly higher frequency in the microbiome of breastfed infants, owing to their ability to rely on Human Milk Oligosacchraides (HMOs) as their sole carbohydrate substrate; namely B. bifidum, B. longum spp. infantis and B. breve. Bifidobacterium kashiwanohense is a species that has been isolated previously only from the faeces of infants, but extremely infrequently at that. Relatively little is currently known about the species itself, let alone the metabolic pathways that allow it to successfully establish a population in the infant gut. We have isolated a novel strain of B. kashiwanohense from the faeces of a breastfed infant on the basis of its ability to utilise the HMO component fucosyllactose as its sole carbohydrate source. In this study, we read and annotate the full genome sequence of this novel strain, and use the data obtained to direct our further experimental analysis of fucosyllactose metabolism in B. kashiwanohense. Using transcriptomic and growth analysis results, we identify the genes responsible for B. kashiwanohense to utilise fucosyllactose, and employ a combination of cloning, in vitro hydrolysis assays, and further, recombinant transcriptomic and growth assays to elucidate the pathway for fucosyllactose metabolism in B. kashiwanohense, as well as revealing insight into fucosyllactose and fucose metabolism in Bifidobacteria as whole.

ORGANISM(S): Bifidobacterium breve UCC2003 Bifidobacterium catenulatum subsp. kashiwanohense

PROVIDER: GSE107439 | GEO | 2019/11/12

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-03-17 | PXD021868 | Pride
2017-02-10 | GSE64017 | GEO
2017-02-10 | GSE63950 | GEO
2014-04-20 | GSE50211 | GEO
2014-05-29 | GSE56291 | GEO
2013-07-01 | GSE36442 | GEO
2020-07-15 | PXD017277 | Pride
2014-04-04 | E-GEOD-56485 | biostudies-arrayexpress
2024-08-27 | PXD047785 | Pride
2014-04-20 | E-GEOD-50211 | biostudies-arrayexpress