CD40 in thymic DC maturation and Treg cell development
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE107571 | GEO | 2017/12/14
SECONDARY ACCESSION(S): PRJNA420736
REPOSITORIES: GEO
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Human lymphoblastoid cells: HLA-DR immunopeptidome identified from different cell dilutions.
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Thymus Files-Thymus 1 (120116WTII), Thymus 2 (020617WT MHCII), Thymus 3 (061417MHCII WT-1), Thymus 4 (061417MHCII WT-2)
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Project description:CD40 in thymic DC maturation and Treg cell development
| PRJNA420736 | ENA
Project description:Analysis of the p38 MAPK pathway in regulation of dendritic cells (DCs) differentiation at the gene expression level. Bone marrow cells were cultured with mGM-CSF (20 ng/ml) in the presence of 1.5 mM of SB202190 or 0.1% DMSO. At day 7, semi-adherent cells were collected as immature DCs (iDCs). iDCs were matured by TNF-a (10 ng/ml) and IL-1b (10 ng/ml) for 48 hours. Results showed that p38 MAPK activity in DC progenitor cells acts as an antigen presentation attenuator, and disabling this critical brake during DC differentiation endows DCs with enhanced immunogenicity, which may be useful for the induction of antitumor immune responses. Total RNA was obtained from 2-day-cultured bone marrow cells, iDCs and mature DCs.
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