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Small RNA Solexa sequencing of mouse hematopoietic progenitor and dendritic cell samples

ABSTRACT: We applied Illumina massively parallel signature sequencing to identify miRNomes in hematopoietic stem cells, bone morrow-derived immature DCs, mature DCs, and IL-10 and NO-producing regulatory DCs.The miRNomes of these DC subsets will contribute to investigate the significance of miRNAs in DC immunobiology. Examination of the miRNome in hematopoietic stem cells, bone morrow-derived immature DCs, mature DCs, and IL-10 and NO-producing regulatory DCs. All four mouse cell types.

ORGANISM(S): Mus musculus

SUBMITTER: Xiaoping Su

PROVIDER: E-GEOD-45882 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1.

Su Xiaoping X Qian Cheng C Zhang Qian Q Hou Jin J Gu Yan Y Han Yanmei Y Chen Yongjian Y Jiang Minghong M Cao Xuetao X

Nature communications 20130101

Dendritic cells (DCs) are critical to initiate the immune response and maintain tolerance, depending on different status and subsets. The expression profiles of microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), which generate DCs, remain to be fully identified. Here we examine miRNomes of mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and histone modification in HSC ...[more]

PMID: 24309499

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