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HIT analysis of T cells activated in the presence of TGF-β

ABSTRACT: We have developed a multi-analyte fluid-phase protein array technology termed high-throughput immunophenotyping using transcription (HIT). This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode. After staining a sample, T7 polymerase amplifies the tags which are then hybridized to a DNA microarray for indirect measurement of each analyte. Here we screened 90 antibodies directed against a panel of cell surface markers as well as 4 isotype controls to compare human naive CD4+ T cells activated in the presence or absence of TGF-β. Keywords: response to stimulus

ORGANISM(S): Homo sapiens

PROVIDER: GSE10762 | GEO | 2008/03/08

SECONDARY ACCESSION(S): PRJNA107547

REPOSITORIES: GEO

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HIT: a new versatile protein array platform for multi-analyte phenotyping of surface molecules

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