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Expression data from Wild-type (WT) and Astrocyte elevated gene-1 (AEG-1) knockout (KO) bone marrow derived macrophages (BMDM)

ABSTRACT: AEG-1 is overexpressed in human hepatocellular carcinoma (HCC) and positively regulates inflammation and development and progression of HCC. An AEG-1KO mouse was generated to understand the function of AEG-1 in inflammatory processes in HCC.

ORGANISM(S): Mus musculus

PROVIDER: GSE107691 | GEO | 2018/12/27

REPOSITORIES: GEO

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Expression data from AEG-1fl/fl and AEG-1DHEP mice which have conditional hepatocyte-specific knockout of AEG-1 (Astrocyte elevated gene 1)

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Expression data from Wild-type (WT) and Alb/AEG-1 mice which are transgenic mice with hepatocyte-specific overexpression of AEG-1 (Astrocyte elevated gene 1)

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Astrocyte elevated gene-1 (AEG-1) promotes hepatocarcinogenesis: novel insights from a mouse model

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Astrocyte elevated gene-1 (AEG-1) promotes hepatocarcinogenesis: novel insights from a mouse model

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Dissecting the Balance between Metabolic and Oncogenic Functions of Astrocyte Elevated Gene-1/Metadherin (AEG-1/MTDH)

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Project description:In vitro studies revealed that AEG-1 is palmitoylated at cysteine at a.a. 75 position. In order to check how palmitoylation regulates AEG-1 function in vivo we generated a knockin moue using CRISPR/Cas9 taking in which cysteine 75 was mutated to serine (AEG-1-C75S). Hepatocytes were isolated from AEG-1-WT and AEG-1C75S littermates, total RNA was isolated and RNA-sequencing was performed.

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