Genomics

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DNA accessibility in CD8+ tumour-infiltrating T cells treated with Phenelzine.


ABSTRACT: Phenelzine treatments were conducted in tumour-bearing Balb/c mice and then 4T1 tumours were acquired. CD8+ tumour-infiltrating T cells were positively selected by using mouse CD8a (Ly-2) microbeads and isolated by autoMACS Separator with recommended programs. Then isolated CD8 T cells were stimulated by PMA and Ionomycin. Global ATAC-seq analysis was performed to identify the dynamic changes in chromatin accessibility of tumour-filtrating CD8 T cells after phenelzine treatment.

ORGANISM(S): Mus musculus

PROVIDER: GSE107724 | GEO | 2018/12/05

REPOSITORIES: GEO

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