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Genome-Wide Identification of ABA Responsive microRNAs in the root and stem of Populus euphratica

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Populus euphratica

PROVIDER: GSE107823 | GEO | 2018/12/01

REPOSITORIES: GEO

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Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

Project description:More then 10 million raw reads were acquired. In total, 238 unique mature sequences which contain 142 conserved miRNA and 96 novel miRNAs were identified.

2018-12-01 | GSE107802 | GEO

Genome-Wide Identification of ABA Responsive microRNAs in the stem of Populus euphratica

Project description:More then 10 million raw reads were acquiredIn total, 247 unique mature sequences which contain 149 conserved miRNA and 98 novel miRNAs were identified.

2018-12-01 | GSE107822 | GEO

Genome-wide characterization of new and drought stress responsive microRNAs in Populus euphratica.

Project description:MicroRNAs (miRNAs) are small, non-coding RNAs that play essential roles in plant growth, development, and stress response. Populus euphratica is a typical abiotic stress-resistant woody species. This study presents an efficient method for genome-wide discovery of new drought stress responsive miRNAs in P. euphratica. High-throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 58 new miRNAs belonging to 38 families were identified, an increase in the number of P. euphratica miRNAs. Twenty-six new and 21 conserved miRNA targets were verified by degradome sequencing, and target annotation showed that these targets were involved in multiple biological processes, including transcriptional regulation and response to stimulus. Furthermore, comparison of high-throughput sequencing with miRNA microarray profiling data indicated that 104 miRNA sequences were up-regulated, whereas 27 were down-regulated under drought stress. This preliminary characterization provides a framework for future analysis of miRNA genes and their roles in key poplar traits such as stress resistance, and could be useful for plant breeding and environmental protection.

| S-EPMC3134338 | biostudies-literature

Genome-wide analysis of salt-responsive and novel microRNAs in Populus euphratica by deep sequencing.

Project description:BackgroundPopulus euphratica is a representative model woody plant species for studying resistance to abiotic stresses such as drought and salt. Salt stress is one of the most common environmental factors that affect plant growth and development. MicroRNAs (miRNAs) are small, noncoding RNAs that have important regulatory functions in plant growth, development, and response to abiotic stress.ResultsTo investigate the miRNAs involved in the salt-stress response, we constructed four small cDNA libraries from P. euphratica plantlets treated with or without salt (300 mM NaCl, 3 days) in either the root or leaf. Using high-throughput sequencing to identify miRNAs, we found 164 conserved miRNAs belonging to 44 families. Of these, 136 novel miRNAs were from the leaf, and 128 novel miRNAs were from the root. In response to salt stress, 95 miRNAs belonging to 46 conserved miRNAs families changed significantly, with 56 miRNAs upregulated and 39 miRNAs downregulated in the leaf. A comparison of the leaf and root tissues revealed 155 miRNAs belonging to 63 families with significantly altered expression, including 84 upregulated and 71 downregulated miRNAs. Furthermore, 479 target genes in the root and 541 targets of novel miRNAs in the leaf were predicted, and functional information was annotated using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases.ConclusionsThis study provides a novel visual field for understanding the regulatory roles of miRNAs in response to salt stress in Populus.

| S-EPMC4118626 | biostudies-literature

Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

Project description:Genome-Wide Identification of ABA Responsive microRNAs in the Root of Populus euphratica

| PRJNA421435 | ENA

Genome-Wide Identification of ABA Responsive microRNAs in the root and stem of Populus euphratica

Project description:Genome-Wide Identification of ABA Responsive microRNAs in the root and stem of Populus euphratica

| PRJNA421492 | ENA

Genome-Wide Identification of ABA Responsive microRNAs in the stem of Populus euphratica

Project description:Genome-Wide Identification of ABA Responsive microRNAs in the stem of Populus euphratica

| PRJNA421493 | ENA

Exploration of ABA Responsive miRNAs Reveals a New Hormone Signaling Crosstalk Pathway Regulating Root Growth of Populus euphratica.

Project description:Abscisic acid (ABA) plays an important role in the regulation of plant adaptation, seed germination, and root development in plants. However, the mechanism of ABA regulation of root development is still poorly understood, especially through the miRNA-mediated pathway. Here, small RNA (sRNA)-seq and degradome-seq were used to analyze the miRNAs' responsive to ABA in the stems and roots of P. euphratica, a model tree species for abiotic stress-resistance research. In total, 255 unique mature sequences, containing 154 known miRNAs and 101 novel miRNAs were identified, among which 33 miRNAs and 54 miRNAs were responsive to ABA in the roots and stems, respectively. Furthermore, the analysis of these miRNAs and their targets revealed a new hormone signaling crosstalk model of ABA regulation of root growth through miRNA-mediated pathways, such as peu-miR-n68 mediation of the crosstalk between ABA and the brassinosteroid (BR) signaling pathway and peu-miR477b mediation of the crosstalk between ABA and Gibberellic acid (GA) signaling. Taken together, our genome-wide analysis of the miRNAs provides a new insight into the mechanism of ABA regulation of root growth in Populus.

| S-EPMC5983633 | biostudies-literature

A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica

Project description:We present an efficient method to genome-wide discover new and drought stress responsive miRNAs in P. euphratica. High throughput sequencing of P. euphratica leaves found 197 conserved miRNAs between P. euphratica and Populus trichocarpa. Meanwhile, 189 new miRNAs which belonged to 120 families were identified, a large increasing to the number of P. euphratica miRNAs. Target prediction and degradome sequencing verification of 22 new and 21 conserved miRNA targets showed these targets were involved in multiple biological processes, including transcription regulation and response to stimulus. Furthermore, comparison of high-throughput sequencing with miRNA microarray profiling data indicated that 104 miRNA sequences were up-regulated, while 27 were down-regulated under drought stress. This preliminary characterization based on our findings provided a framework for future analysis of miRNA genes and their roles in key traits of poplar as stress resistance plant breeding and environment protection usage.

2011-04-25 | GSE25747 | GEO

A Genome-Wide Characterization of New and Drought Stress Responsive MicroRNAs in Populus euphratica

2011-04-25 | E-GEOD-25747 | biostudies-arrayexpress