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LMP1 mediated gene expression changes in transfected CD10+ GC B cells

ABSTRACT: In this study, we have investigated the effect of LMP1 on gene expression in normal human GC B cells using a non-viral vector based system Keywords: transfection of viral oncogene in normal human B cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE10821 | GEO | 2008/10/10

SECONDARY ACCESSION(S): PRJNA107423

REPOSITORIES: GEO

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LMP1 mediated gene expression changes in transfected CD10+ GC B cells

Project description:In this study, we have investigated the effect of LMP1 on gene expression in normal human GC B cells using a non-viral vector based system Experiment Overall Design: Gene expression was compared between LMP1-transfected and control vector-transfected GC B cells from three patients. RNA from the FACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using significance analysis of microarrays (SAM) with a 1.5 fold change threshold and the q-value threshold set to 5%.

2008-10-26 | E-GEOD-10821 | biostudies-arrayexpress

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Project description:In this study, we have investigated the effect of LMP2A on gene expression in normal human GC B cells using a non-viral vector based system Keywords: transfection of viral oncogene in normal human B cells Gene expression was compared between LMP2A-transfected and control vector-transfected GC B cells from three patients. RNA from the FACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed probe sets were identified using limma with a fold change >1.3 and p < 0.01

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Project description:In this study, we have investigated the effect of BLIMP1α on gene expression, cell differentiation and pathogenesis in normal human GC B cells using a non-viral vector based system Gene expression was compared among BLIMP1α, LMP1-transfected and control vector-transfected GC B cells from two patients. RNA from the FACS-sorted transfected GC B cells was amplified. Fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using the GCOS pair-wise analysis.

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Down-regulation of BLIMP1-alpha by the EBV oncogene, LMP1, disrupts the plasma cell differentiation program and prevents viral replication in B cells

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Transcriptome analysis for LMP1 depletion rescue with Wildtype LMP1 or individual TES mutant LMP1 in GM12878 Lymphoblastoid cell line (LCL)

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