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Expression profiling of HONE1 cell line stably transfected with LMP1

ABSTRACT: Using gene expression profiling technology from Capitalbio to find out the whole genome influence after LMP1 stably transfectted to HONE1 cell line.

ORGANISM(S): Homo sapiens

PROVIDER: GSE18867 | GEO | 2009/11/05

SECONDARY ACCESSION(S): PRJNA121121

REPOSITORIES: GEO

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Expression profiling of HONE1 cell line stably transfected with LMP1

Project description:Using gene expression profiling technology from Capitalbio to find out the whole genome influence after LMP1 stably transfectted to HONE1 cell line. We analyzed gene expression change by LMP1 in HONE1 cell to find the active pathway and related biology function.

2010-06-20 | E-GEOD-18867 | biostudies-arrayexpress

Transcriptome analysis for LMP1 depletion rescue with Wildtype LMP1 or individual TES mutant LMP1 in GM12878 Lymphoblastoid cell line (LCL)

Project description:RNA-seq was used to characterize the LMP1 mediated regulation of host target gene regulation. Here, we stably expressed doxycycline-induced HA-tagged wildtype (WT) LMP1 or TES1 mutant (TES1m) oe TES2 mutant(TES2m) in GM12878 LCL. We then depleted LMP1 from these cells using CRISPR-Cas9 approach and treated the cells with 400ng/ml of doxycycline to induce expression of WT, TES1m or TES2m LMP1 to rescue from the LMP1 depletion mediated effects (including loss of cell viability) to understand and differenciate the role of LMP1 TES1 vs TES2 domains in LCL.

2023-08-25 | GSE240732 | GEO

Analysis of microRNA expression during Epstein-Barr virus encoded LMP1 and LMP2A transfected in TW03 cells

Project description:Epstein-Barr virus has been reported to regulate cellular microRNA expression in B cells. In the present study, we investigated the differential microRNAs modulated by Epstein-Barr virus in Naspharyngeal Carcinoma, using CapitalBio corporation's mammalian miRNA arrays. Three cellular models were used in this study: the human naspharyngeal carcinoma cell line TW03 as a blank control; TW03 transfected with Epstein-Barr virus encoded LMP1; TW03 transfected with Epstein-Barr virus encoded LMP2A

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Project description:Differential methylation between human normal fibroblast cell CCD-18Co transfected with P16-Dnmt or pTRIPZ control vector was identified using Illumina HumanMethylation 850K array. The genome-wide methylation detection experiment was carried out by CapitalBio Technology Company.

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Homo sapiens

Project description:Expression profiling of HONE1 cell line stably transfected with LMP1

| PRJNA121121 | ENA

LMP1 mediated gene expression changes in transfected CD10+ GC B cells

Project description:In this study, we have investigated the effect of LMP1 on gene expression in normal human GC B cells using a non-viral vector based system Experiment Overall Design: Gene expression was compared between LMP1-transfected and control vector-transfected GC B cells from three patients. RNA from the FACS-sorted transfected GC B cells was amplified. 10ug of fragmented cRNA was hybridized to HG-U133 Plus 2.0 microarrays. Differentially expressed genes were identified using significance analysis of microarrays (SAM) with a 1.5 fold change threshold and the q-value threshold set to 5%.

2008-10-26 | E-GEOD-10821 | biostudies-arrayexpress

Transcriptome analysis for LMP1 depletion in GM12878 Lymphoblastoid cell line (LCL)

Project description:RNA-seq was used to characterize the LMP1 mediated regulation of host target gene regulation. We knocked out LMP1 in GM12878 Lymphoblastoid cell line (LCL). The GM12878 LCL expressing control sgRNA was used as the control.

2023-04-25 | GSE228167 | GEO

LMP1 mediated gene expression changes in transfected CD10+ GC B cells

Project description:In this study, we have investigated the effect of LMP1 on gene expression in normal human GC B cells using a non-viral vector based system Keywords: transfection of viral oncogene in normal human B cells

2008-10-10 | GSE10821 | GEO

Analysis of microRNA expression during Epstein-Barr virus encoded LMP1 and LMP2A transfected in TW03 cells

2011-01-13 | GSE26596 | GEO

Epstein-Barr Virus (EBV) Latent Membrane Protein 1 (LMP1) Regulates Host B Cell miR-155 and its Target FOXO3a via PI3K p110a Activation

Project description:The purpose of this study was to investigate the host cell miRs regulated by LMP1 from the B95-8 strain of EBV. In this study, we utilized an established chimeric, inducible NGFRLMP1 siganling molecule stably expressed in EBV- BL41 Burkitt’s Lymphoma cell lines (BL41 NGFRLMP1 cells). A qPCR array was used to measure the expression level of 377 previously identified miRs from RNA isolated from two sources: BL41 NGFRLMP1 cells in which LMP1 signaling was not activated (n=2, control) and BL41 NGFRLMP1 cells in which LMP1 signaling was activated for 12 hours (n=2).

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