Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

ABSTRACT: RNAseq were performed to analyze the transcriptomic differences between healthy homeostatic MSC, pre-leukemic MSC and recovered MSC.

ORGANISM(S): Mus musculus

PROVIDER: GSE108725 | GEO | 2020/12/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vivo]

Project description:RNAseq were performed to analyze the transcriptomic differences between macrophage and MSC-educated macrophages in vivo.

2020-12-29 | GSE125028 | GEO

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vitro]

Project description:RNAseq were performed to analyze the transcriptomic differences between macrophages and MSC-educated macrophages in vitro.

2020-12-29 | GSE125027 | GEO

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-29 | GSE125029 | GEO

Secretome analysis of human bone marrow derived mesenchymal stromal cells

Project description:As an essential cellular component of the bone marrow (BM) microenvironment mesenchymal stromal cells (MSC) play a pivotal role for the physiological regulation of hematopoiesis, in particular through the secretion of cytokines and chemokines. Mass spectrometry (MS) facilitates the identification and quantification of a large amount of secreted proteins (secretome), but can be hampered by the false-positive identification of contaminating proteins released from dead cells or derived from cell medium. To reduce the likelihood of contaminations we applied an approach combining secretome and proteome analysis to characterize the physiological secretome of BM derived human MSC. Our analysis revealed a secretome consisting of 315 proteins. Pathway analyses of these proteins revealed a high abundance of proteins related to cell growth and/or maintenance, signal transduction and cell communication thereby representing key biological functions of BM derived MSC on protein level. Within the MSC secretome we identified several cytokines and growth factors such as VEGFC, TGF-β1, TGF-β2 and GDF6 which are known to be involved in the physiological regulation of hematopoiesis. By comparing the peptide patterns of secretomes and cell lysates 17 proteins were identified as candidates for proteolytic processing. Taken together, our combined MS work-flow reduced the likelihood of contaminations and enabled us to carve out a specific overview about the composition of the secretome from human BM derived MSC. This methodological approach and the specific secretome signature of BM derived MSC may serve as basis foffuture comparative analyses of the interplay of MSC and HSPC in patients with hematological malignancies.

2019-02-12 | PXD011643 | Pride

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

| PRJNA514994 | ENA

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

| PRJNA428374 | ENA

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vitro]

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vitro]

| PRJNA514997 | ENA

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vivo]

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vivo]

| PRJNA514996 | ENA

Gene expression profiles of human Mesenchymal Stromal Cells (MSC) from JAK2+ myeloproliferative neoplasms (MPN)

Project description:In this study we analyzed the behavior of bone marrow MSC (BM-MSC) from MPN patients with the mutation in JAK2V617F. We initially characterized the biological function and gene expression profile changes in BM-MSC from MPN patients when compared to BM-MSC of healthy donors (HD). Then, we established co-cultures between MSC cell lines (HTERT and HS5) and the UKE-1 MPN cell line, and performed RT-PCR to study if the leukemic cells were able to modify the genes related to hematopoietic support.

2017-09-14 | GSE87806 | GEO

miRNOME of healthy donor and myelodysplastic bone marrow mesenchymal stromal cells (MSC)

Project description:We have demonstrated that DICER1 (a key enzyme of miRNA biogenesis) was underexpressed in myelodysplastic syndrome MSC. We hypothesize that those MSC harbored a deregulated pattern of miRNA versus healthy donors MSC.

2020-10-04 | GSE139471 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data