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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vitro]

ABSTRACT: RNAseq were performed to analyze the transcriptomic differences between macrophages and MSC-educated macrophages in vitro.

ORGANISM(S): Mus musculus

PROVIDER: GSE125027 | GEO | 2020/12/29

REPOSITORIES: GEO

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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vivo]

Project description:RNAseq were performed to analyze the transcriptomic differences between macrophage and MSC-educated macrophages in vivo.

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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

Project description:RNAseq were performed to analyze the transcriptomic differences between healthy homeostatic MSC, pre-leukemic MSC and recovered MSC.

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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-29 | GSE125029 | GEO

Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vitro]

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vitro]

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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages

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Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vivo]

Project description:Mesenchymal stromal cells orchestrate the overhaul of leukemia bone marrow microenvironment via educating macrophages [in vivo]

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Secretome analysis of human bone marrow derived mesenchymal stromal cells

Project description:As an essential cellular component of the bone marrow (BM) microenvironment mesenchymal stromal cells (MSC) play a pivotal role for the physiological regulation of hematopoiesis, in particular through the secretion of cytokines and chemokines. Mass spectrometry (MS) facilitates the identification and quantification of a large amount of secreted proteins (secretome), but can be hampered by the false-positive identification of contaminating proteins released from dead cells or derived from cell medium. To reduce the likelihood of contaminations we applied an approach combining secretome and proteome analysis to characterize the physiological secretome of BM derived human MSC. Our analysis revealed a secretome consisting of 315 proteins. Pathway analyses of these proteins revealed a high abundance of proteins related to cell growth and/or maintenance, signal transduction and cell communication thereby representing key biological functions of BM derived MSC on protein level. Within the MSC secretome we identified several cytokines and growth factors such as VEGFC, TGF-β1, TGF-β2 and GDF6 which are known to be involved in the physiological regulation of hematopoiesis. By comparing the peptide patterns of secretomes and cell lysates 17 proteins were identified as candidates for proteolytic processing. Taken together, our combined MS work-flow reduced the likelihood of contaminations and enabled us to carve out a specific overview about the composition of the secretome from human BM derived MSC. This methodological approach and the specific secretome signature of BM derived MSC may serve as basis foffuture comparative analyses of the interplay of MSC and HSPC in patients with hematological malignancies.

2019-02-12 | PXD011643 | Pride

Bone Marrow Microenvironment in Light-Chain Amyloidosis: In Vitro Expansion and Characterization of Mesenchymal Stromal Cells.

Project description:Immunoglobulin light-chain amyloidosis (AL) is caused by misfolded light chains produced by a small B cell clone. Mesenchymal stromal cells (MSCs) have been reported to affect plasma cell behavior. We aimed to characterize bone marrow (BM)-MSCs from AL patients, considering functional aspects, such as proliferation, differentiation, and immunomodulatory capacities. MSCs were in vitro expanded from the BM of 57 AL patients and 14 healthy donors (HDs). MSC surface markers were analyzed by flow cytometry, osteogenic and adipogenic differentiation capacities were in vitro evaluated, and co-culture experiments were performed in order to investigate MSC immunomodulatory properties towards the ALMC-2 cell line and HD peripheral blood mononuclear cells (PBMCs). AL-MSCs were comparable to HD-MSCs for morphology, immune-phenotype, and differentiation capacities. AL-MSCs showed a reduced proliferation rate, entering senescence at earlier passages than HD-MSCs. The AL-MSC modulatory effect on the plasma-cell line or circulating plasma cells was comparable to that of HD-MSCs. To our knowledge, this is the first study providing a comprehensive characterization of AL-MSCs. It remains to be defined if the observed abnormalities are the consequence of or are involved in the disease pathogenesis. BM microenvironment components in AL may represent the targets for the prevention/treatment of the disease in personalized therapies.

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miRNOME of healthy donor and myelodysplastic bone marrow mesenchymal stromal cells (MSC)

Project description:We have demonstrated that DICER1 (a key enzyme of miRNA biogenesis) was underexpressed in myelodysplastic syndrome MSC. We hypothesize that those MSC harbored a deregulated pattern of miRNA versus healthy donors MSC.

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