Transcriptomics

Dataset Information

0

The satellite cell niche regulates the balance between myoblast differentiation and self-renewal via p53


ABSTRACT: Satellite cells (SCs) are adult muscle stem cells residing in a specialised niche that regulates SC homeostasis. How niche-generated signals integrate to regulate gene expression in SC-derived myoblasts, is poorly understood. We undertook an unbiased approach to study the effect of the SC niche on SC-derived myoblast transcriptional regulation and identified the tumour suppressor p53 as a key player in the regulation of myoblast quiescence. After activation and proliferation, a subpopulation of myoblasts cultured in the presence of the niche upregulates p53 and fails to differentiate. When SC self-renewal is modelled ex vivo in a reserve cell assay, myoblasts treated with Nutlin-3, which increases p53 levels in the cell, fail to differentiate and instead become quiescent. Since both these effects of Nutlin-3 are rescued by siRNA-mediated p53 knockdown we conclude that a tight control of p53 levels in myoblasts regulate the balance between differentiation and return to quiescence.

ORGANISM(S): Mus musculus

PROVIDER: GSE109052 | GEO | 2018/01/11

SECONDARY ACCESSION(S): PRJNA429482

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-07-05 | E-GEOD-20150 | biostudies-arrayexpress
2008-04-09 | E-GEOD-10435 | biostudies-arrayexpress
2021-11-11 | PXD029693 |
2022-12-01 | GSE215627 | GEO
2022-06-11 | GSE205690 | GEO
2018-12-31 | GSE110742 | GEO
| PRJNA434444 | ENA
2018-10-24 | PXD002413 | Pride
2022-03-04 | GSE182421 | GEO
2005-12-09 | GSE3780 | GEO