Methylation profiling

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RNA bisulfite sequencing of candidate tRNAs in Drosophila melanogaster


ABSTRACT: Dnmt2 and NSun2 are (cytosine-5) RNA methyltransferases. Using CRISPR/Cas9 we created null mutations in Dnmt2 and NSun2 genes in Drosophila melanogaster. We also ectopically expressed a wild type and catalytically inactive Dnmt2 form in the Dnmt2 mutant background. RNA bisulfite sequencing was used to follow RNA methylation at partical tRNA substrates.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE109133 | GEO | 2018/01/16

SECONDARY ACCESSION(S): PRJNA430044

REPOSITORIES: GEO

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