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Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

ABSTRACT: We report the application of RNA-sequencing technology for high-throughput profiling of tumor-associated microglia/macrophage (TAMs). First, we checked the purity of sample by qRT-PCR. We confirmed the high purity of TAMs with rare other cell type contamination. By profiling samples of TAMs purified from five individual medulloblastoma of mouse model, we find that TAMs maintain microglia gene signature even there are decrease of microglia genes and increase of macrophage genes. This study provides the evidence for the application of comprehensive gene profiling towards characterization of cellular origin of cell populations in tumor microenvironment.

ORGANISM(S): Mus musculus

PROVIDER: GSE109750 | GEO | 2019/12/31

REPOSITORIES: GEO

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Astrocytic trans-Differentiation Completes a Multicellular Paracrine Feedback Loop Required for Medulloblastoma Tumor Growth.

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Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

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Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

Project description:Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

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Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

Project description:Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth

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CSF-1R inhibition alters macrophage polarization and blocks gliomagenesis

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analysis of the immune and astrocytic cell fraction 12h after cortical injury in Sema4B knockout mice

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