Genomics

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Direct DNA crosslinking with CAP-C uncovers transcription-dependent chromatin organization at high resolution


ABSTRACT: We introduce Chemical-crosslinking Assisted Proximity Capture (CAP-C), a method that utilizes multi-functional chemical crosslinkers with defined physical sizes to directly assess spatial distances between nuclear genomic sequences. CAP-C avoids limitations caused by extensive protein-DNA crosslinking in most in situ methods, and thus generates chromatin contact maps at high resolution with low background noise.

ORGANISM(S): Mus musculus Homo sapiens Drosophila melanogaster

PROVIDER: GSE110061 | GEO | 2020/08/22

REPOSITORIES: GEO

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