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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

ABSTRACT: Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAProtect, total RNA purification, and microRNA expression analysis against clone-, patient-, and passage-matched ground control samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE110561 | GEO | 2018/07/26

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

Project description:Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAprotect. Gene expression analysis was performed against clone- and passage-matched ground control samples.

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

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Microarray Analysis of Space-flown Murine Thymus Tissue

Project description:Microarray Analysis of Space-flown Murine Thymus Tissue Reveals Changes in Gene Expression Regulating Stress and Glucocorticoid Receptors. We used microarrays to detail the gene expression of space-flown thymic tissue and identified distinct classes of up-regulated genes during this process. We report here microarray gene expression analysis in young adult C57BL/6NTac mice at 8 weeks of age after exposure to spaceflight aboard the space shuttle (STS-118) for a period of 13 days. Upon conclusion of the mission, thymus lobes were extracted from space flown mice (FLT) as well as age- and sex-matched ground control mice similarly housed in animal enclosure modules (AEM). mRNA was extracted and an automated array analysis for gene expression was performed. Examination of the microarray data revealed 970 individual probes that had a 1.5 fold or greater change. When these data were averaged (n=4), we identified 12 genes that were significantly up- or down-regulated by at least 1.5 fold after spaceflight (p≤0.05). Together, these data demonstrate that spaceflight induces significant changes in the thymic mRNA expression of genes that regulate stress, glucocorticoid receptor metabolism, and T cell signaling activity. These data explain, in part, the reported systemic compromise of the immune system after exposure to the microgravity of space.

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-07-26 | GSE110563 | GEO

Transcriptomic Effects on the Mouse Heart Following 30 Days on the International Space Station

Project description:Efforts to understand the impact of spaceflight on the human body stem from the growing interest in long-term space travel. Multiple organ systems are affected by micro-gravity and radiation, including the cardiovascular system. Previous transcriptomic studies have sought to reveal the changes in gene expression after spaceflight. However, little is known about the impact of long-term spaceflight on the mouse heart in vivo. This study focused on transcriptomic changes in the hearts of female C57BL/6J mice flown on the International Space Station (ISS) for 30 days. RNA was isolated from the hearts of three flight and three comparable ground control mice and RNA sequencing was performed. Our analysis showed that 1,147 transcripts were significantly regulated after spaceflight. MAPK, PI3K-Akt and GPCR signaling pathways were predicted to be activated. Transcripts related to cytoskeleton breakdown and organization were upregulated, but no significant change in the expression of extracellular matrix (ECM) components or oxidative stress pathway associated transcripts occurred. Our results indicate an absence of cellular senescence, and a significant upregulation of transcripts associated with the cell cycle. Transcripts related to cellular maintenance and survival were most affected by spaceflight, suggesting that the cardiovascular transcriptome initiates an adaptive response to long-term spaceflight

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Influence of International Space Station Conditions on Fungal ‘Omics’ in Aspergillus nidulans

Project description:This study presents the first global genomic, proteomic, and secondary metabolomic characterization of the filamentous fungus, Aspergillus nidulans, following growth on the International Space Station (ISS). The investigation included the A. nidulans wild-type and 3 mutant strains, two of which were genetically engineered to enhance secondary metabolite (SM) production. Whole genome sequencing (WGS) revealed that ISS conditions altered the A. nidulans genome in specific regions. In strain CW12001, which features overexpression of the SM global regulator laeA, ISS conditions induced a point mutation that resulted in the loss of the laeA stop codon. Differential expression of proteins involved in stress response, carbohydrate metabolic processes, and SM biosynthesis was observed. ISS conditions significantly decreased prenyl xanthone production in the wild-type strain and increased asperthecin production in LO1362 and CW12001, which are deficient in a major DNA repair mechanism. Together, these data provide valuable insights into the genetic and molecular adaptation mechanism of A. nidulans to the spacecraft environment and present many economic benefits.

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