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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

ABSTRACT: Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

PROVIDER: PRJNA433985 | ENA |

REPOSITORIES: ENA

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

Project description:Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAProtect, total RNA purification, and microRNA expression analysis against clone-, patient-, and passage-matched ground control samples.

2018-07-26 | GSE110561 | GEO

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

Project description:Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAprotect. Gene expression analysis was performed against clone- and passage-matched ground control samples.

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

Project description:Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

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Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

Project description:This SuperSeries is composed of the SubSeries listed below.

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Effect of a 91 day long stay in weightlessness on the International Space Station on mouse skin physiology

Project description:Comparitive gene expression in skin between mice maintained in microgravity (0g) and normogravity (1g) environment. Six male C57Bl/J10 mice were housed for 91 days in the specially designed \Mouse Drawer System\ in weightlessness aboard the International Space Station. Three wild-type mice (WT) and three transgenic mice overexpressing the osteogneic factor PTN/OSF1 under the control of the human bone specific ostecalcin promoter (Tg) were used in the experiment. During the 3-month stay on the ISS, 3 mice unfortunately died leaving 2 Tg and 1 WT. \MDS tissue sharing program\ allowed several teams to study various tissues from these mice. Our aim was to investigate the effect of such a long period of microgravity on skin physiology by morphological, biochemical and genomewide analyses by comparison to similar mice on ground. Gene expression in the skin of 3 space mice and of 3 ground mice was analyzed by microarray. As this unique experiment performed on 3 mice limits the power of statistical analyis, as the transgene PTN/OSF1 was not overexpressed in skin and as a pair wise Pearson's correlation rates between the individual levels of expressed transcripts in the WT and the Tg mice were not significantly different from each other in one experimental group (space or ground), data from the 3 mice were combined to compare results from the space an ground groups.

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A CRISPR-based assay for the study of eukaryotic DNA repair aboard the International Space Station

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Transcriptomic Effects on the Mouse Heart Following 30 Days on the International Space Station

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