Other

Dataset Information

0

A CRISPR loss-of-function Hepatic progenitor cells expansion screen in liver organoids


ABSTRACT: Biliary epithelial cells (BECs) form bile ducts in the liver, and are facultative liver stem cells that establish a ductular reaction (DR) to support liver regeneration following injury. Liver damage induces periportal LGR5+ putative liver stem cells which can form BEC-like organoids, suggesting RSPO-LGR4/5-mediated WNT/β-catenin activity is important for a DR. We addressed the roles for this and other signaling pathways in a DR by performing a focused CRISPR-based loss-of-function screen in BEC-like organoids, followed by in vivo validation and single-cell RNA sequencing. We found BECs lack and do not require LGR4/5-mediated WNT/β-Catenin signaling during DR, while YAP and mTORC1 signaling are required for this process. Upregulation of LGR5/AXIN2 is required in hepatocytes to enable their regenerative capacity in response to injury. Together, these data highlight heterogeneity within the BEC pool and delineate signaling pathways involved.

ORGANISM(S): Mus musculus synthetic construct

PROVIDER: GSE111269 | GEO | 2018/08/27

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-07-04 | E-GEOD-28265 | biostudies-arrayexpress
2023-03-22 | GSE217739 | GEO
2024-09-29 | PXD056355 |
2013-01-28 | E-GEOD-32210 | biostudies-arrayexpress
2011-07-04 | GSE28265 | GEO
2012-03-10 | E-GEOD-27904 | biostudies-arrayexpress
2012-03-10 | GSE27904 | GEO
2016-07-15 | E-GEOD-84456 | biostudies-arrayexpress
| EGAS00001006808 | EGA
2013-09-17 | E-GEOD-50103 | biostudies-arrayexpress