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Bisulfite-free, nano-scale analysis of 5-hydroxymethylcytosine at single base resolution

ABSTRACT: High-resolution detection of genome-wide 5-hydroxymethylcytosine (5hmC) sites of small-scale samples represents a continuous challenge. Here, we present CATCH-seq, a bisulfite-free, base-resolution method for the genome-wide detection of 5hmC. CATCH-seq is based on selective 5hmC oxidation, labeling and subsequent C-to-T transition during PCR. Applications of CATCH-seq to nano-scale DNA samples reveal previously underappreciated non-CG 5hmCs in the hESC genome and base-resolution hydroxymethylome in human cell-free DNA.

ORGANISM(S): Homo sapiens

PROVIDER: GSE112048 | GEO | 2018/10/09

REPOSITORIES: GEO

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High-resolution enzymatic mapping of genomic 5-hydroxymethylcytosine in mouse embryonic stem cells

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