Bisulfite-free and Base-resolution Analysis of 5-formylcytosine at Whole-genome Scale
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ABSTRACT: Active DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxycytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present a bisulfite-free method for the whole-genome analysis of 5fC, based on a selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps reveal limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.
ORGANISM(S): Mus musculus
PROVIDER: GSE66144 | GEO | 2015/09/07
SECONDARY ACCESSION(S): PRJNA275968
REPOSITORIES: GEO
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