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Analysis of gene expression in co-culture of PBMCs with breast cancer cell lines by High-Throughput Sequencing

ABSTRACT: The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on breast cancer cell lines. Transcription profiles of PBMCs (control) and PBMCs co-cultured with breast cancer cell lines (treatment) were generated by deep sequencing, in triplicate for MCF7 and duplicate for T47D, using Illumina HiSeq 4000. Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with breast cancer cell lines. The result revealed significantly up- and down-regulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE112280 | GEO | 2021/12/31

REPOSITORIES: GEO

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Analysis of gene expression in co-culture of PBMCs with colon cancer cell lines by High-Throughput Sequencing

Project description:(Objectives) The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on colon cancer cell lines. (Method) Transcription profiles of PBMCs (control) and PBMCs co-cultured with colon cancer cell lines (treatment) were generated by deep sequencing, in triplicate for SW480 and duplicate for HT29, using Illumina HiSeq 4000. (Results and conclusion) Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with colon cancer cell lines. The result revealed significantly up- and down-regulated genes.

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Analysis of gene expression in co-culture of PBMCs with Head and Neck cancer cells line by High-Throughput Sequencing

Project description:(Objectives) The goal of this study is to investigate paracrine effect of Peripheral Blood Mononuclear Cells (PBMCs) on Head and Neck cancer cell lines. (Method) Transcription profiles of PBMCs (control) and PBMCs co-cultured with Head and Neck cancer cell lines (treatment) were generated by deep sequencing, in triplicate for HN17 and duplicate for HN4, using Illumina HiSeq 4000. (Results and conclusion) Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with HN cell lines. The result revealed significantly down-regulated genes. On the other hand, up-regulated gene, ZCCHC6 were used to validated with qRT-PCR in HNSCC patient blood. The synopsis of this study demonstrates paracrine effect on PBMC of HNSCC patient to significantly up expression of ZCCHC6 gene when compared with healthy donors.

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Analysis of gene expression in co-culture of PBMCs with lung cancer cell lines by High-Throughput Sequencing

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2021-12-31 | GSE112005 | GEO

Analysis of gene expression in co-culture of PBMCs with Hepatocellular carcinoma by High-Throughput Sequencing

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Project description:We cultured human lung and breast cancer cell lines, A549, H23, MDA-MB-231, MCF-7-TNR, and T47D cells. Total RNA was extracted and processed for RNA-SEQ.

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PBMCs from metastatic breast cancer patients

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| PRJNA730015 | ENA

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