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Active ribosome profiling with RiboLace and standard ribosome profiling in HEK-293 cells

ABSTRACT: We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes by means of an antibody-free and tag-free pull-down approach. RiboLace is fast, it works with very low input material and can be easily and rapidly used to enhance Ribo-Seq, obtaining a global snapshot on active ribosome footprints at single nucleotide resolution from eukaryotic in vitro and in vivo systems. Keywords: ribosome profiling, translation, RiboLace, Ribo-Seq, active translation

ORGANISM(S): Homo sapiens

PROVIDER: GSE112353 | GEO | 2018/10/09

REPOSITORIES: GEO

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