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Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila

ABSTRACT: Transcriptomic analyses of wild type and Domino knockdown 3rd instar larval neural cells

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE112395 | GEO | 2018/05/15

REPOSITORIES: GEO

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Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila

Project description:Stem cells establish cortical polarity and divide asymmetrically to simultaneously maintain themselves and generate differentiating offspring cells. Several chromatin modifiers have been identified as stemness factors in mammalian pluripotent stem cells, but whether these factors control stem cell polarity and asymmetric division has not been investigated so far. We addressed this question in Drosophila neural stem cells called neuroblasts. We identified the Tip60 chromatin remodeling complex and its interaction partner Myc to regulate target genes required for neuroblast maintenance. Knockdown of members of this complex results in loss of cortical polarity, symmetric neuroblast division and premature differentiation through nuclear entry of the transcription factor Prospero. We found that aPKC is the key target gene of Myc and the Tip60 complex subunit Domino regulating neuroblast polarity. Our transcriptome analysis further showed that Domino regulates the expression of mitotic spindle genes which were identified before as direct Myc targets. Our findings reveal an evolutionarily conserved functional link between Myc, the Tip60 complex and the molecular network controlling cell polarity and asymmetric cell division.

2018-07-06 | PXD009939 | Pride

Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila.

Project description:Stem cells establish cortical polarity and divide asymmetrically to simultaneously maintain themselves and generate differentiating offspring cells. Several chromatin modifiers have been identified as stemness factors in mammalian pluripotent stem cells, but whether these factors control stem cell polarity and asymmetric division has not been investigated so far. We addressed this question in Drosophila neural stem cells called neuroblasts. We identified the Tip60 chromatin remodeling complex and its interaction partner Myc as regulators of genes required for neuroblast maintenance. Knockdown of Tip60 complex members results in loss of cortical polarity, symmetric neuroblast division, and premature differentiation through nuclear entry of the transcription factor Prospero. We found that aPKC is the key target gene of Myc and the Tip60 complex subunit Domino in regulating neuroblast polarity. Our transcriptome analysis further showed that Domino regulates the expression of mitotic spindle genes previously identified as direct Myc targets. Our findings reveal an evolutionarily conserved functional link between Myc, the Tip60 complex, and the molecular network controlling cell polarity and asymmetric cell division.

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D. melanogaster eye-antennal imaginal discs developmental transcriptome

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Myc and the Tip60 chromatin remodeling complex control neuroblast maintenance and polarity in Drosophila

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RNA-Seq of whole Drosophila larval central nervous systems (mid-3rd instar stage, central brain + ventral nerve cord) with panneuronal ISWI knockdown and controls

Project description:Sleep disruptions are among the most commonly-reported symptoms across neurodevelopmental disorders (NDDs), but mechanisms linking brain development to normal sleep are largely unknown. From a Drosophila screen of human NDD-associated risk genes, we identified the chromatin remodeler Imitation SWItch/SNF (ISWI) to be required for adult fly sleep. To better understand the mechanisms by which ISWI regulates sleep, we performed RNA-Seq analysis on mid-3rd instar larval central nervous systems (central brain + ventral nerve cord) in the setting of pan-neuronal ISWI knockdown (elav-GAL4>UAS-ISWI RNAi) compared to controls (elav-GAL4;+). We chose mid-3rd instar because temporal mapping revealed ISWI knockdown during this pre-adult developmental stage led to adult fly sleep deficits.

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Identification of (E)-β-farnesene impair the development and survival of Chilo suppressalis larval by disrupting insect hormone balance

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mRNA microarray of Drosophila melanogaster extratced from cephalic complexes of lgl27S3/lglE2S31 (lgl-null) and FRT82B (wild-type) 3rd instar larvae

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MicroRNA microarray of Drosophila melanogaster extracted from cephalic complexes of lgl-hypomorph 3rd instar larvae

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Expression data from larvae body wall expressing LaminC transgenes

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