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MRNA of fed and starved C. elegans L1 larvae 3 generations following control, short-term dauer or long-term dauer conditions

ABSTRACT: N2 worms were subjected to control (well-fed), short-term dauer (6 d), or long-term dauer (40 - 49 d) conditions. Worms were recovered and maintained well-fed for 3 generations. F3 progeny were collected as fed or starved L1 larvae.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE113500 | GEO | 2018/07/27

REPOSITORIES: GEO

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mRNA of fed and starved C. elegans L1 larvae 3 generations following control, short-term dauer or long-term dauer conditions

Project description:mRNA of fed and starved C. elegans L1 larvae 3 generations following control, short-term dauer or long-term dauer conditions

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Project description:Transcriptional profiling of P. pacificus worms from (1) dauer stage, or (2) dauer-exit at 12 hours stage, compared to mix-stage worms as a common reference. The goal was to determine genes regulated during dauer development and recovery or exit from dauer stage. This data was then compared to data generated for corresponding developmental stages in the C. elegans (see NCBI GEO series GSE30977) , to study evolution of developmental pathways regulating dauer development. Two-condition experiments. Experiment 1 = Dauers vs. Mix-stage worms. 4 biological replicates for each condition, including 2 dye-swaps. Experiment 2 = Dauer-Exit at 12 hour time-point s vs. Mix-stage worms. 3 biological replicates for each condition, including 1 dye-swaps. Total samples from both experiments 1 and 2 = 7.

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P. pacificus : Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

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C. elegans: Dauers and Dauer-Exit at 12 hour time-point vs. Mix-stage worms

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