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Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [Agilent]

ABSTRACT: Purpose: To understand how gene expression changes in yan and pnt mutant embryos

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE114208 | GEO | 2018/05/08

REPOSITORIES: GEO

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Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [ChIP-Seq]

Project description:Purpose: To understand how the Drosophila ETS transcription factors Yan and Pointed together with the corepressor Groucho are recruited to chromatin

2018-05-08 | GSE114092 | GEO

Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila.

Project description:The acquisition of cellular identity during development depends on precise spatiotemporal regulation of gene expression, with combinatorial interactions between transcription factors, accessory proteins and the basal transcription machinery together translating complex signaling inputs into appropriate gene expression outputs. The opposing repressive and activating inputs of the Drosophila ETS family transcription factors Yan and Pointed orchestrate numerous cell fate transitions downstream of receptor tyrosine kinase signaling, providing one of the premier systems for studying this process. Current models describe the differentiative transition as a switch from Yan-mediated repression to Pointed-mediated activation of common target genes. We describe here a new layer of regulation whereby Yan and Pointed co-occupy regulatory elements to repress gene expression in a coordinated manner, with Pointed being unexpectedly required for the genome-wide occupancy of both Yan and the co-repressor Groucho. Using even skipped as a test-case, synergistic genetic interactions between Pointed, Groucho, Yan and components of the RNA polymerase II pausing machinery suggest that Pointed integrates multiple scales of repressive regulation to confer robustness. We speculate that this mechanism may be used broadly to fine-tune the expression of many genes crucial for development.

| S-EPMC6053666 | biostudies-literature

Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-05-08 | GSE114209 | GEO

Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila

Project description:Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila

| PRJNA470552 | ENA

Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [Agilent]

Project description:Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [Agilent]

| PRJNA470554 | ENA

Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [ChIP-Seq]

Project description:Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness in Drosophila [ChIP-Seq]

| PRJNA464289 | ENA

Cooperative recruitment of Yan via a high-affinity ETS supersite organizes repression to confer specificity and robustness to cardiac cell fate specification.

Project description:Cis-regulatory modules (CRMs) are defined by unique combinations of transcription factor-binding sites. Emerging evidence suggests that the number, affinity, and organization of sites play important roles in regulating enhancer output and, ultimately, gene expression. Here, we investigate how the cis-regulatory logic of a tissue-specific CRM responsible for even-skipped (eve) induction during cardiogenesis organizes the competing inputs of two E-twenty-six (ETS) members: the activator Pointed (Pnt) and the repressor Yan. Using a combination of reporter gene assays and CRISPR-Cas9 gene editing, we suggest that Yan and Pnt have distinct syntax preferences. Not only does Yan prefer high-affinity sites, but an overlapping pair of such sites is necessary and sufficient for Yan to tune Eve expression levels in newly specified cardioblasts and block ectopic Eve induction and cell fate specification in surrounding progenitors. Mechanistically, the efficient Yan recruitment promoted by this high-affinity ETS supersite not only biases Yan-Pnt competition at the specific CRM but also organizes Yan-repressive complexes in three dimensions across the eve locus. Taken together, our results uncover a novel mechanism by which differential interpretation of CRM syntax by a competing repressor-activator pair can confer both specificity and robustness to developmental transitions.

| S-EPMC5900712 | biostudies-literature

Genome-Wide Yan Chromatin Occupancy in Drosophila Embryos

Project description:The ETS transcriptional repressor Yan functions as part of a developmental switch that in response to receptor tyrosine kinase signaling, transitions from a high-Yan to a low-Yan state to direct specification of a variety of cell fates. To date very few direct target genes have been identified, nor is it clear how their expression is buffered against developmental noise to prevent inappropriate oscillations between states. Following investigation of its genome-wide chromatin occupancy profile, we noticed a striking signature at developmentally important genes whereby Yan associates with chromatin in regions of high-peak density that span multiple kilobases which partially relies upon SAM-domain mediated self-association. We speculate that the high-density Yan occupancy signature may reveal a novel mechanism that buffers the expression of critical developmental regulators against intrinsic and environmental noise. The supplementary bed file contains Yan binding regions. Yan ChIP from staged Drosophila embryos

2013-03-01 | E-GEOD-34038 | biostudies-arrayexpress

Genome-Wide Yan Chromatin Occupancy in Drosophila Embryos

2013-03-01 | GSE34038 | GEO

Genome-wide maps of Yan chromatin association

Project description:High-resolution genome-wide binding of Yan used to confirm the presence of high-density regions seen in ChIP-chip ChIP-Seq of Yan protein in stage 11 Drosophila embryos

2013-03-01 | E-GEOD-34040 | biostudies-arrayexpress

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