Transcriptomics

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NONO knockdown RNA-seq and neuroblastoma PARCLIP analysis of NONO


ABSTRACT: siRNA knockdown of NONO mRNA followed by RNA-seq was carried out in the KELLY neuroblasotma cell line. In order to determine potential regulatory targets of NONO protein, we performed differential expression analysis. To identify the RNA targets of NONO in neuroblastoma, we carried out PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) using an antibody recognising NONO protein.

ORGANISM(S): Homo sapiens

PROVIDER: GSE114376 | GEO | 2023/01/28

REPOSITORIES: GEO

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