Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (ChIP-seq)

ABSTRACT: ChIP-seq of SWI/SNF subunits and histone modifications in RWPE1 cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE114392 | GEO | 2018/05/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (RIP-seq)

Project description:RNA immunoprecipitation of SWI/SNF in prostate cacner

2018-05-14 | GSE114393 | GEO

SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (ATAC-seq)

Project description:Changes to chromatin openness were measured by ATAC seq in RWPE1 cells expression SCHLAP1 or a control cell line infected with a LACZ lentivirus.

2018-05-14 | GSE114391 | GEO

SWI/SNF remains localized to chromatin in the presence of SCHLAP1.

Project description:SCHLAP1 is a long noncoding RNA that is reported to function by depleting the SWI/SNF complex from the genome. We investigated the hypothesis that SCHLAP1 affects only specific compositions of SWI/SNF. Using several assays, we found that SWI/SNF is not depleted from the genome by SCHLAP1 and that SWI/SNF is associated with many coding and noncoding RNAs, suggesting that SCHLAP1 may function in a SWI/SNF-independent manner.

| S-EPMC6339527 | biostudies-literature

SWI/SNF remains localized to chromatin in the presence of SCHLAP1

Project description:SWI/SNF remains localized to chromatin in the presence of SCHLAP1

| PRJNA471271 | ENA

SWI/SNF remains localized to chromatin in the presence of SCHLAP1

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-05-14 | GSE114394 | GEO

SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (ChIP-seq)

Project description:SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (ChIP-seq)

| PRJNA471288 | ENA

SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (RIP-seq)

Project description:SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (RIP-seq)

| PRJNA471291 | ENA

SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (ATAC-seq)

Project description:SWI/SNF remains localized to chromatin in the presence of SCHLAP1 (ATAC-seq)

| PRJNA471289 | ENA

Genome-wide profile of the SWI/SNF chromatin remodeling complex

Project description:A systems understanding of nuclear organization and events is critical for determining how cells divide, differentiate and respond to stimuli and for identifying the causes of diseases. Chromatin remodeling complexes such as SWI/SNF have been implicated in a wide variety of cellular processes including gene expression, nuclear organization, centromere function and chromosomal stability, and mutations in SWI/SNF components have been linked to several types of cancer. To better understand the biological processes in which chromatin remodeling proteins participate we globally mapped binding regions for several components of the SWI/SNF complex throughout the human genome using ChIP-Seq. SWI/SNF components were found to lie near regulatory elements integral to transcription (e.g. 5M-bM-^@M-^Y ends, RNA Polymerases II and III and enhancers) as well as regions critical for chromosome organization (e.g. CTCF, lamins and DNA replication origins). To further elucidate the association of SWI/SNF subunits with each other as well as with other nuclear proteins we also analyzed SWI/SNF immunoprecipitated complexes by mass spectrometry. Individual SWI/SNF factors are associated with their own family members as well as with cellular constituents such as nuclear matrix proteins, key transcription factors and centromere components implying a ubiquitous role in gene regulation and nuclear function. We find an overrepresentation of both SWI/SNF-associated regions and proteins in cell cycle and chromosome organization. Taken together the results from our ChIP and immunoprecipitation experiments suggest that SWI/SNF facilitates gene regulation and genome function more broadly and through a greater diversity of interactions than previously appreciated. ChIP-Seq analysis of the SWI/SNF subunits Ini1, Brg1, BAF155 and BAF170 in HeLa S3 cells

2011-01-10 | E-GEOD-24397 | biostudies-arrayexpress

Composition and Function of Mutant Swi/Snf Complexes.

Project description:The 12-subunit Swi/Snf chromatin remodeling complex is conserved from yeast to humans. It functions to alter nucleosome positions by either sliding nucleosomes on DNA or evicting histones. Interestingly, 20% of all human cancers carry mutations in subunits of the Swi/Snf complex. Many of these mutations cause protein instability and loss, resulting in partial Swi/Snf complexes. Although several studies have shown that histone acetylation and activator-dependent recruitment of Swi/Snf regulate its function, it is less well understood how subunits regulate stability and function of the complex. Using functional proteomic and genomic approaches, we have assembled the network architecture of yeast Swi/Snf. In addition, we find that subunits of the Swi/Snf complex regulate occupancy of the catalytic subunit Snf2, thereby modulating gene transcription. Our findings have direct bearing on how cancer-causing mutations in orthologous subunits of human Swi/Snf may lead to aberrant regulation of gene expression by this complex.

2017-08-07 | MSV000081418 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data