Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing Facilitates Quantitative Analysis of three groups from mouse liver Transcriptomes

ABSTRACT: Liver mRNA profiles of WT liver(WT1-WT3) and Alb-Cre:Hdac3−/− liver (0d(Ha-Hc), 7d(Hd-Hg), 21d(Hh-Hk), 50d(Hl-Hn)) receiving successive PH(sPH), Alb-Cre:Hdac3−/− tumor tissues(HCC1-HCC3) and adjacent non-tumor tissues(N1-N3), spontaneous liver cancers of Alb-Cre:Hdac3-/- mice (HCC1-HCC3) and liver tumor of recipient Fah-/- mice (HCCa-HCCc) were generated by deep sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE114503 | GEO | 2021/05/11

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microarray analysis of WT, SKO, PKO and DKO liver samples

Project description:Microarray analysis of WT (Pten2fl/fl:Shp2fl/fl:Alb-Cre-), SKO (Shp2hep-/-, or Shp2fl/fl:Alb-Cre+), PKO (Ptenhep-/-, or Pten2fl/fl:Alb-Cre+) and DKO (Ptenfl/fl:Shp2fl/fl:Alb-Cre+) liver samples to gain global molecular insights how shp2 and pten is involved in liver tumorigenesis.

2016-12-13 | GSE74732 | GEO

Gene expression profiling of HDAC3-depleted mouse liver rescued with wild-type exogenous HDAC3

Project description:We have shown that intravenous injection of HDAC3 floxed mice with adeno-associated virus (AAV) expressing Cre depletes hepatic HDAC3, upregulates lipogenic gene expression, and causes fatty liver. When AAV-Flag-HDAC3 wild-type (WT) is co-injected along with AAV-Cre, the exogenous HDAC3 is expressed at endogenous levels and can completely rescue fatty liver phenotype. Here we profile transcriptome of the rescued WT livers in comparison with HDAC3-depleted (KO) livers. 4-months old C57BL/6 male mice were co-injected with AAV-Cre or AAV-Cre plus AAV-Flag-HDAC3. Mice were fed ad libitum and harvested at 5 pm (ZT10) at 2-weeks post-injection. Liver total RNA was extracted and hybridized to Affymetrix Mouse Gene 1.0ST array.

2013-11-25 | E-GEOD-49386 | biostudies-arrayexpress

Expression data from WT SIRT6 and SIRT6 albumin CRE conditional mice

Project description:Microarray analysis of liver tissue from WT SIRT6 and conditional knockout of SIRT6 using albumin-Cre (SIRT6Co/Co ;Alb-Cre) at 2 and 8 months of age RNA was extracted from mouse liver tissue at 2 and 8 months of age. RNA from three pairs of WT SIRT6 and SIRT6Co/Co ;Alb-Cre mice was combined and hybridized to Affymetrix mouse gene 1.0 ST arrays.

2010-06-08 | E-GEOD-21965 | biostudies-arrayexpress

Differential gene expression of mouse liver samples after poly I:C treatment

Project description:To understand differential gene expression globally with/without poly I:C treatment on liver cancer mouse model, RNA-seq analysis were examined using diethlynitrosamine-induced Shp2∆H (Shp2F/F:Alb-cre) and Pten∆H (PtenF/F:Alb-cre) mice.

2017-10-25 | GSE95782 | GEO

Gene expression profile in the small Maf-deficient livers.

Project description:To elucidate the functional roles of sMafs in the adult liver, we conditionally targeted the sMaf genes using a transgenic complementation rescue approach. MafF-/-::MafG-/-::MafK-/- (F0G0K0) mice are embryonic lethal but can be rescued by complementation of transgenic MafG expression under the regulation of the MafG regulatory domain (MGRD). Therefore, we rescued F0G0K0 mice using a MGRD transgenic mouse line with a MafG gene flanked with loxP (fMafG) sequences so that the MafG gene could be deleted by Cre-mediated recombination. The Albumin(Alb)-Cre transgenic mice were used to delete fMafG gene specifically in the liver. The genotype used are MafF-/-::MafG-/-::MafK-/-::MGRD-fMafG::Alb-Cre (liver-specific sMaf CKO) and MafF-/-::MafG+/-::MafK-/-::MGRD-fMafG::Alb-Cre (control).

2017-05-01 | GSE84001 | GEO

Comprehensive proteometics identifies FBXL6 substrates involved in liver cancer

Project description:FBXL6 is frequently over-expressed in human hepatocellular carcinoma (HCC). However, it is still unknown the underlying mechanisms by which FBXL6 promotes HCC. In this study, we compared ubiquitinated protein profiles among a panel of liver tissue samples (including HCC, adjacent tissues and normal tissues) from Fbxl6LSL-fl/+; Alb-cre mice and Alb-cre mice by proteomics and ubiquitomics analysis. There are many proteins with ubiquitination in FBXL6 overexpressed HCC, suggesting ubiquitination may play a critical role in FBXL6-mediated HCC. A1--- normal tissue 1 A2--- normal tissue 2 B1--- Adjacent tissue 1 B2--- Adjacent tissue 2 C1--- HCC tissue 1 C2--- HCC tissue 2

2024-01-26 | PXD032357 | Pride

Gene expression profiling of HDAC3-depleted mouse liver rescued with wild-type exogenous HDAC3

2013-11-25 | GSE49386 | GEO

Gene expression in mouse liver depleted of HDAC3

Project description:Liver-specific depletion of HDAC3 leads to liver steatosis (fatty liver), suggesting disregulation of lipid metabolism. This is correlated with changes in lipid metabolic gene expression. Livers depleted of HDAC3 were removed from 12 week old male HDAC3 fl/fl mice (loxP sites flanking exon 4 to 7 of the HDAC3 gene encoding the catalytic domain of HDAC3) one week after the injection of AAV2/8-Tbg-Cre virus. Livers from the HDAC3 fl/fl mice injected with AAV2/8-Tbg-GFP were used as normal controls. mRNA was extracted from 100mg mouse liver samples and hybridized to Affymetrix microarrays. For each group (HDAC3 depleted liver and normal liver), we have 5 samples from different mice.

2011-03-11 | E-GEOD-25937 | biostudies-arrayexpress

Expression data from WT SIRT6 and SIRT6 albumin CRE conditional mice

Project description:Microarray analysis of liver tissue from WT SIRT6 and conditional knockout of SIRT6 using albumin-Cre (SIRT6Co/Co ;Alb-Cre) at 2 and 8 months of age

2010-05-28 | GSE21965 | GEO

Next Generation Sequencing Analysis of Wild Type and hnRNPK-MKO liver transcription

Project description:we report liver fibrosis defects in hnRNPK Alb-Cre mice, in which hnRNPK is ablated in hepatocyte cells.

2024-06-10 | GSE176594 | GEO