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Drosophila melanogaster late third instar larval testes and gonadal fatbody RNA-seq profiling

ABSTRACT: In order to study the effect of protease treatment on dissected testes, we performed different strategies of cleaning and dissociation on Drosophila melanogaster w1118 larval testes. We collected testes without protease treatment with fatbody just attached around the gonad, fatbody alone dissected from around the testes, testes without fatbody and testes dissociated by either by Papain or Trypsin and Collagenase cocktail. We prepared poly A+ RNA-Seq libraries and performed transcriptional profiling to generate 50 bp stranded single end reads.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE115478 | GEO | 2018/06/08

REPOSITORIES: GEO

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