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Gene expression after HOS cells treated with DGKZ (diacylglycerol kinase zeta)-silencing by a microarray profiling (Affymetrix)

ABSTRACT: DGKZ could promote proliferation and inhibit apoptosis in osteosarcoma,both in vivo and in vitro. To identify downstream molecular mechanism of DGKZ in proliferation of osteosarcoma, we performed a microarray profiling (Affymetrix) in human HOS cells treated with DGKZ knockdown.

ORGANISM(S): Homo sapiens

PROVIDER: GSE115590 | GEO | 2018/06/12

REPOSITORIES: GEO

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Gene expression after HOS cells treated with DGKZ (diacylglycerol kinase zeta)-silencing by a microarray profiling (Affymetrix)

Project description:Gene expression after HOS cells treated with DGKZ (diacylglycerol kinase zeta)-silencing by a microarray profiling (Affymetrix)

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2012-05-22 | E-GEOD-38133 | biostudies-arrayexpress

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2012-05-22 | E-GEOD-38134 | biostudies-arrayexpress

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Changes in gene expression in HOS and U2-OS cell lines after exposure to 600 Î¼g/mL OGE

Project description:Osteosarcoma (Osteosarcoma) is a type of bone cancer. Eighty percent of this tumor will be metastatic to the lungs or liver, and as a result, patients generally need chemotherapy to improve survival possibility. Recently, anti-tumor activity has been reported in Ocimum gratissimum aqueous extract (OGE), which has been the focus of recent extensive studies on therapeutic strategies due to its antioxidant properties. We used microarrays to identify potential and novel target genes responsive to the anticancer effect in OGE treatment in osteosarcoma cells, We performed pharmacogenomics analyses for the effect of OGE on human osteosarcoma U2-OS and HOS cell growth. Cell viability, Western blot and flow cytometry analysis were performed before performing pharmacogenomics analyses for the effect of OGE on human osteosarcoma U2-OS and HOS cell growth, including cDNA microarray and RT-PCR assays.

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Diacylglycerol kinase ζ controls diacylglycerol metabolism at the immunological synapse.

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