Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

ABSTRACT: We demonstrate that actin interacts with essentially all transcribed genes and co-occupies most gene promoters together with RNA polymerase II (Pol II). On highly expressed genes, actin and Pol II can be found also on the gene bodies.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE116362 | GEO | 2018/08/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Nuclear actin is required for transcription during Drosophila oogenesis

Project description:Nuclear actin is required for transcription during Drosophila oogenesis

| PRJNA478253 | ENA

Nuclear Actin Is Required for Transcription during Drosophila Oogenesis.

Project description:Actin has been linked to processes spanning the whole gene expression cascade, from regulating specific transcription factors, such as myocardin-related transcription factor, to chromatin remodeling and RNA polymerase function. However, whether actin controls the transcription of only specific genes or has a global role in gene expression has remained elusive. Our genome-wide analysis reveals, for the first time, that actin interacts with essentially all transcribed genes in Drosophila ovaries. Actin co-occupies the majority of gene promoters together with Pol II, and on highly expressed genes, these two proteins also associate with gene bodies. Mechanistically, actin is required for Pol II recruitment to gene bodies, and manipulation of nuclear transport factors for actin leads to the decreased expression of eggshell genes. Collectively, these results uncover a global role for actin in transcription and demonstrate the in vivo importance of balanced nucleocytoplasmic shuttling of actin in the transcriptional control of a developmental process.

| S-EPMC6214840 | biostudies-literature

Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

Project description:Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

| PRJNA478251 | ENA

Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

Project description:Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

| PRJNA478255 | ENA

Nuclear actin is required for transcription during Drosophila oogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-08-01 | GSE116365 | GEO

Actin associates with actively elongating genes

Project description:Nuclear actin has been demonstrated to be essential for optimal transcription, but the molecular mechanisms and direct binding partner for actin in the RNA polymerase complex have remained unknown. By using purified proteins in several biochemical assays, we demonstrate a direct and specific interaction between monomeric actin and Cdk9, the kinase subunit of the positive transcription elongation factor b (P-TEFb) required for RNA polymerase II (Pol II) pause-release. This interaction is not dependent on kinase activity of Cdk9 and is not involved with releasing P-TEFb from its inhibitor 7SK snRNP complex. Supporting the specific role for actin in the elongation phase of transcription, chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) reveals that actin interacts with genes only upon their active transcription elongation. This study therefore provides novel insights into the mechanisms by which actin facilitates the transcription process.

2023-09-01 | GSE239771 | GEO

Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

Project description:we performed transcriptomic analysis of the RanBP9del1 mutant ovaries compared to wild type

2018-08-01 | GSE116363 | GEO

Fascin regulates nuclear actin during Drosophila oogenesis.

Project description:Drosophila oogenesis provides a developmental system with which to study nuclear actin. During Stages 5-9, nuclear actin levels are high in the oocyte and exhibit variation within the nurse cells. Cofilin and Profilin, which regulate the nuclear import and export of actin, also localize to the nuclei. Expression of GFP-tagged Actin results in nuclear actin rod formation. These findings indicate that nuclear actin must be tightly regulated during oogenesis. One factor mediating this regulation is Fascin. Overexpression of Fascin enhances nuclear GFP-Actin rod formation, and Fascin colocalizes with the rods. Loss of Fascin reduces, whereas overexpression of Fascin increases, the frequency of nurse cells with high levels of nuclear actin, but neither alters the overall nuclear level of actin within the ovary. These data suggest that Fascin regulates the ability of specific cells to accumulate nuclear actin. Evidence indicates that Fascin positively regulates nuclear actin through Cofilin. Loss of Fascin results in decreased nuclear Cofilin. In addition, Fascin and Cofilin genetically interact, as double heterozygotes exhibit a reduction in the number of nurse cells with high nuclear actin levels. These findings are likely applicable beyond Drosophila follicle development, as the localization and functions of Fascin and the mechanisms regulating nuclear actin are widely conserved.

| S-EPMC5042582 | biostudies-literature

Nuclear Actin Regulates Inducible Transcription by Enhancing RNA Polymerase II Clustering

Project description:Nuclear actin dynamics regulate enhanced RNA polymerase II clustering on specific genes upon serum stimulation, thus establishing the serum-induced transcriptional program.

2017-12-01 | GSE82320 | GEO

Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation

Project description:Nuclear myosin 1c (NM1) is emerging as regulator of transcription and chromatin organization. Using a genome-wide approach we report here that NM1 binds across the mammalian genome with occupancy peaks at class II gene promoters, correlating with distributions of RNA Polymerase II (Pol II) and active epigenetic marks. We show that NM1 synergizes with polymerase-associated actin to maintain active Pol II at gene promoters. NM1 also co-localizes with the nucleosome remodeler SNF2h at class II promoters where they assemble together with WSTF as part of the B-WICH complex to remodel chromatin. Following B-WICH assembly, NM1 mediates physical recruitment of the histone acetyl transferase PCAF and the histone methyl transferase Set1/Ash2 to maintain and preserve H3K9acetylation and H3K4trimethylation for active transcription. We propose a novel genome-wide mechanism where myosin synergizes with Pol II-associated actin to link the polymerase machinery with permissive chromatin for transcription activation. Association of nuclear myosin 1 (NM1) with the genome in mouse embryonic fibroblasts

2015-05-04 | E-GEOD-66542 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data