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Nuclear actin is required for transcription during Drosophila oogenesis

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE116365 | GEO | 2018/08/01

REPOSITORIES: GEO

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Nuclear Actin Is Required for Transcription during Drosophila Oogenesis.

Project description:Actin has been linked to processes spanning the whole gene expression cascade, from regulating specific transcription factors, such as myocardin-related transcription factor, to chromatin remodeling and RNA polymerase function. However, whether actin controls the transcription of only specific genes or has a global role in gene expression has remained elusive. Our genome-wide analysis reveals, for the first time, that actin interacts with essentially all transcribed genes in Drosophila ovaries. Actin co-occupies the majority of gene promoters together with Pol II, and on highly expressed genes, these two proteins also associate with gene bodies. Mechanistically, actin is required for Pol II recruitment to gene bodies, and manipulation of nuclear transport factors for actin leads to the decreased expression of eggshell genes. Collectively, these results uncover a global role for actin in transcription and demonstrate the in vivo importance of balanced nucleocytoplasmic shuttling of actin in the transcriptional control of a developmental process.

| S-EPMC6214840 | biostudies-literature

Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

Project description:we performed transcriptomic analysis of the RanBP9del1 mutant ovaries compared to wild type

2018-08-01 | GSE116363 | GEO

Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

Project description:We demonstrate that actin interacts with essentially all transcribed genes and co-occupies most gene promoters together with RNA polymerase II (Pol II). On highly expressed genes, actin and Pol II can be found also on the gene bodies.

2018-08-01 | GSE116362 | GEO

Fascin regulates nuclear actin during Drosophila oogenesis.

Project description:Drosophila oogenesis provides a developmental system with which to study nuclear actin. During Stages 5-9, nuclear actin levels are high in the oocyte and exhibit variation within the nurse cells. Cofilin and Profilin, which regulate the nuclear import and export of actin, also localize to the nuclei. Expression of GFP-tagged Actin results in nuclear actin rod formation. These findings indicate that nuclear actin must be tightly regulated during oogenesis. One factor mediating this regulation is Fascin. Overexpression of Fascin enhances nuclear GFP-Actin rod formation, and Fascin colocalizes with the rods. Loss of Fascin reduces, whereas overexpression of Fascin increases, the frequency of nurse cells with high levels of nuclear actin, but neither alters the overall nuclear level of actin within the ovary. These data suggest that Fascin regulates the ability of specific cells to accumulate nuclear actin. Evidence indicates that Fascin positively regulates nuclear actin through Cofilin. Loss of Fascin results in decreased nuclear Cofilin. In addition, Fascin and Cofilin genetically interact, as double heterozygotes exhibit a reduction in the number of nurse cells with high nuclear actin levels. These findings are likely applicable beyond Drosophila follicle development, as the localization and functions of Fascin and the mechanisms regulating nuclear actin are widely conserved.

| S-EPMC5042582 | biostudies-literature

Nuclear actin is required for transcription during Drosophila oogenesis

Project description:Nuclear actin is required for transcription during Drosophila oogenesis

| PRJNA478253 | ENA

Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

Project description:Nuclear actin is required for transcription during Drosophila oogenesis [ChIP-seq]

| PRJNA478251 | ENA

Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

Project description:Nuclear actin is required for transcription during Drosophila oogenesis [RNA-seq]

| PRJNA478255 | ENA

Genome organization regulates nuclear pore complex formation and promotes differentiation during Drosophila oogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-05-29 | GSE250351 | GEO

Polycomb function during oogenesis is required for mouse early embryonic development

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-05-07 | GSE28711 | GEO

Direct interaction between two actin nucleators is required in Drosophila oogenesis.

Project description:Controlled actin assembly is crucial to a wide variety of cellular processes, including polarity establishment during early development. The recently discovered actin mesh, a structure that traverses the Drosophila oocyte during mid-oogenesis, is essential for proper establishment of the major body axes. Genetic experiments indicate that at least two proteins, Spire (Spir) and Cappuccino (Capu), are required to build this mesh. The spire and cappuccino genetic loci were first identified as maternal effect genes in Drosophila. Mutation in either locus results in the same phenotypes, including absence of the mesh, linking them functionally. Both proteins nucleate actin filaments. Spir and Capu also interact directly with each other in vitro, suggesting a novel synergistic mode of regulating actin. In order to understand how and why proteins with similar biochemical activity would be required in the same biological pathway, genetic experiments were designed to test whether a direct interaction between Spir and Capu is required during oogenesis. Indeed, data in this study indicate that Spir and Capu must interact directly with one another and then separate to function properly. Furthermore, these actin regulators are controlled by a combination of mechanisms, including interaction with one another, functional inhibition and regulation of their protein levels. Finally, this work demonstrates for the first time in a multicellular organism that the ability of a formin to assemble actin filaments is required for a specific structure.

| S-EPMC4007717 | biostudies-literature

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