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The induction of core pluripotency master regulators in cancers defines poor clinical outcomes and treatment resistance

ABSTRACT: Clinical expression of core pluripotent stem cell master regulators OCT4, SOX2 and NANOG is associated with treatment resistance and lethal cancers. We have described a novel model to induce the expression of these factors in vitro. We term this culture environment ‘Acquired Pluripotent Stem Cell Environment (APSCE)’ and RNA-seq comparisons were made to culture in conventional serum supplemented ‘Full Media’ (FM) in human prostate (LNCaP, CWR22Rv1) and bladder (RT112) cancer cell lines. Depletion of the androgen receptor (AR) with siRNA targeting exon1(siEX1) was additionally performed in the CWR22Rv1 cell line alongside a scrambled control (siCTRL).

ORGANISM(S): Homo sapiens

PROVIDER: GSE117193 | GEO | 2019/02/25

REPOSITORIES: GEO

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