Identification of differentially expressed miRNAs in exosomes secreted from human colon cancer stem cells.
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE117479 | GEO | 2021/07/01
REPOSITORIES: GEO
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Project description:To study if the transcriptional content in exosomes derived from unntreated and growth factor treated cultured cardiomyocytes (HL-1) differ and if so, can this difference be explained.This is results for the cells.The exosome reults can be find at GSE40503. 4 control untreated samples, 4 TFG-beta2 cardiomyocyte treated samples and 4 PDGF BB cardiomyocyte were studied., this reults are from the corresponding cells (that released the exosomes), used for data filtering of the exsomedata. The exosome reults can be find at GSE40503.
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2011-10-24 | E-GEOD-33179 | biostudies-arrayexpress
Project description:Dendritic cells (DCs) are the most potent antigen (Ag)-presenting cells. Whereas immature DCs down-regulate T cell responses to induce/maintain immunological tolerance, mature DCs promote immunity. To amplify their functions, DCs communicate with neighboring DCs through soluble mediators, cell-to-cell contact and vesicle exchange. Transfer of nanovesicles (<100nm) derived from the endocytic pathway (termed exosomes) represents a novel mechanism of DC-to-DC communication. The facts that exosomes contain exosome-shuttle microRNAs (miRNAs), and DC functions can be regulated by exogenous miRNAs, suggest that DC-to-DC interactions could be mediated through exosome-shuttle miRNAs, an hypothesis that remains to be tested. Importantly, the mechanism of transfer of exosome-shuttle miRNAs from the exosome lumen to the cytosol of target cells is unknown. Here, we demonstrate that DCs release exosomes with different miRNAs depending on the maturation of the DCs. By visualizing spontaneous transfer of exosomes between DCs, we demonstrate that exosomes fused with the target DCs, the latter followed by release of the exosome content into the DC cytosol. Importantly, exosome-shuttle miRNAs are functional, as they repress target mRNAs of acceptor DCs. Our findings unveil a mechanism of transfer of exosome-shuttle miRNAs between DCs and its role as a means of communication and post-transcriptional regulation between DCs.
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