Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Genome-wide expression analysis of the effects of a parasitic nematode's secreted exosomes on mammalian cells.

ABSTRACT: We show that a gastrointestinal nematode that infects mice, Heligmosomoides polygyrus, secretes vesicles packaged with specific microRNAs (miRNAs) and Y RNAs as well as a nematode-specific Argonaute protein. The vesicles are of intestinal origin and are enriched for homologs of mammalian proteins found in exosomes, including heat shock proteins, tetraspanins and an ESCORT protein. The nematode-derived vesicles are internalized by mouse intestinal epithelial cells and mediate changes in expression of host genes involved in inflammation and immunity, including the receptor for the alarmin IL33 as well as a key regulator of MAPK signaling, DUSP1. A total of 12 samples were analyzed, from 4 different conditions: medium, exosome-treated, LPS and LPS+exosomes, each with 3 biological replicates. One of the 'medium' replicates was discarded due to outlier behaviour.

ORGANISM(S): Mus musculus

SUBMITTER: Cei Abreu-Goodger

PROVIDER: E-GEOD-55941 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity.

Buck Amy H AH Coakley Gillian G Simbari Fabio F McSorley Henry J HJ Quintana Juan F JF Le Bihan Thierry T Kumar Sujai S Abreu-Goodger Cei C Lear Marissa M Harcus Yvonne Y Ceroni Alessandro A Babayan Simon A SA Blaxter Mark M Ivens Alasdair A Maizels Rick M RM

Nature communications 20141125

In mammalian systems RNA can move between cells via vesicles. Here we demonstrate that the gastrointestinal nematode Heligmosomoides polygyrus, which infects mice, secretes vesicles containing microRNAs (miRNAs) and Y RNAs as well as a nematode Argonaute protein. These vesicles are of intestinal origin and are enriched for homologues of mammalian exosome proteins. Administration of the nematode exosomes to mice suppresses Type 2 innate responses and eosinophilia induced by the allergen Alternari ...[more]

PMID: 25421927

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Dataset Information

Genome-wide expression analysis of the effects of a parasitic nematode's secreted exosomes on mammalian cells.

Publications

Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity.

Similar Datasets

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