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Genome-wide maps of heterochromatin state (H3K9me2) in kinetochore and other centromere related mutants.

ABSTRACT: We report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 maps of fission yeast mutants with either deletions of non-essential kinetochore genes or conditional inactivation (temperature sensitive, ts) mutations in essential ones. We find that the impairment of the kinetochore componnets cause various levels (from no to prominent) of heterochroamatin spreading into centromeric core regions. Hence, we conclude that the integrity of the inner kinetochore is required to maintain normal centromeric chromatin organization as well as distinct centromere identity.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE117948 | GEO | 2019/09/28

REPOSITORIES: GEO

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