Genomics

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Genome-wide maps of chromatin state (H3K9me2 or CENP-A/Cnp1) in wt strains carrying the inactivated Centromere 1 or 2 or Cnp1 spreading with ura4 inserted into the right side of the repetitive sequences of Chromosome 1 (designated as otr1R::ura4)


ABSTRACT: We report the high-throughput profiling of histone modification (H3K9me2) or histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 or CENP-A/Cnp1 maps of wild type strains carrying the inactivated Centromere 1 or Centromere 2 or Cnp1 spreading in fission yeast with a reporter gene ura4 cassette inserted into the right side of the repetitive sequences of Chromosome 1 . We find that neocemtromeres are formed preferably at pericentromeric regions ubiquitously and asymmetrically in all three centromeres of these indicated strains.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE131225 | GEO | 2019/09/28

REPOSITORIES: GEO

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