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Genetic interaction analysis of RSA1 deletion strains (GIM screen)

ABSTRACT: A S. cerevisiae strain with deletion of RSA1 gene was combined with two collections of yeast mutants: one in which non-essential genes were deleted and one in which an long 3' UTR extension has been added to the mRNA of essential genes (GIM method, Decourty et al., 2008). Combined double-mutant deletion cells growth was quantified using barcodes that are specific for each gene mutation. A reference population was obtained by mixing the results of 15 GIM screens DNA prior to barcode amplification and labeling (Decourty et al., in preparation). The identification of genetic interactions between deletion of RSA1 and deletion of RTT106, a histone chaperone, indicate a potential role of Rtt106 in snoRNP formation.

ORGANISM(S): Saccharomyces cerevisiae S288C Saccharomyces cerevisiae

PROVIDER: GSE118550 | GEO | 2019/10/10

REPOSITORIES: GEO

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