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Single cell RNA Sequencing analysis on CD45 positive cells isolated form Lewis Lung Carcinoma (LLC) tumors.

ABSTRACT: The goal of this study is to compare transcriptome profiling of different populations of CD45 immune cells of isolated from LLC tumors from miR-21 conditionally deleted on LysM expressing cells (mir-21 flox/flox; lysmCre) mice vs. control (mir-21flox/flox) mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE118931 | GEO | 2019/11/11

REPOSITORIES: GEO

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RNA Sequencing analysis on Tumor associated macrophages (TAMs) Isolated form Lewis Lung Carcinoma (LLC) tumors

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Expression data from the subclones of Lewis Lung Carcinoma (LLC) cells

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Single cell RNA Sequencing analysis on CD45 positive cells isolated form Lewis Lung Carcinoma (LLC) tumors.

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Project description:We previously identified an extracellular pH-sensing G protein-coupled receptor T cell death associated gene 8 (TDAG8) as an extracellular pH sensor of tumor cells that promotes cell growth/survival in vitro and tumor development in vivo. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with total RNA prepared from these cells. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with a mixture of equal amounts of total RNA samples from four independent cell cultures.

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