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Microarray analysis of T-cell death-associated gene 8 (TDAG8)-mediated gene expression in mouse Lewis lung carcinoma (LLC) cells.

ABSTRACT: We previously identified an extracellular pH-sensing G protein-coupled receptor T cell death associated gene 8 (TDAG8) as an extracellular pH sensor of tumor cells that promotes cell growth/survival in vitro and tumor development in vivo. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with total RNA prepared from these cells. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with a mixture of equal amounts of total RNA samples from four independent cell cultures.

ORGANISM(S): Mus musculus

SUBMITTER: Satoshi Ishii

PROVIDER: E-GEOD-31776 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray analysis of T-cell death-associated gene 8 (TDAG8)-mediated gene expression in mouse Lewis lung carcinoma (LLC) cells.

Project description:We previously identified an extracellular pH-sensing G protein-coupled receptor T cell death associated gene 8 (TDAG8) as an extracellular pH sensor of tumor cells that promotes cell growth/survival in vitro and tumor development in vivo. To determine genes regulated by TDAG8 in vitro, mouse Lewis lung carcinoma (LLC) cells stably expressing TDAG8 (TDAG8-LLC cells) and control vector (control LLC cells) were cultured under acidic conditions. Transcriptome microarray analysis using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays was performed with total RNA prepared from these cells.

2012-11-01 | GSE31776 | GEO

Visualization of cancer initiating cell in the vascular niche utilized with transcriptional activity of PSF1

Project description:Identification of cancer stem/initiating cells (CSCs/CICs) by a specific marker is useful for diagnosis and therapy of cancer. We have determined that PSF1 which plays a role in DNA replication in lower species is strongly expressed in wide range of normal stem cell population. Here, utilizing the transcriptional activity of PSF1 promoter in tumor cell xenograft model, we show that PSF1high cancer cells display malignant features including high proliferating activity, serial transplantation potential, and metastatic ability those are used for criteria of CSCs/CICs. PSF1high cancer cells localize in perivascular region and genetically display ES cell like signature. Silencing of PSF1 by RNAi inhibited growth of cancer cells mediated by disruption of DNA synthesis and chromosomal segregation. These suggested that PSF1 is a possible maker and a molecular target of CSCs/CICs. We have cloned the mouse PSF1 promoter gene and established lung carcinoma (lewis lung carcinoma: LLC) and colon cancer (colon26) cell lines stably expressing enhanced green fluorescent protein (EGFP) under the transcriptional control of PSF1 promoter (LLC- and colon-PSF1P-EGFP, respectively). Mouse xenograft tumor cells were dissociated and sorted into PSF1 low and high expression cells by FACS. We sought whether PSF1 high expression cells possess embryonic stem cells-like signature.

2010-04-12 | E-GEOD-17112 | biostudies-arrayexpress

Comparative study of 3D vs. 2D cell culture transcriptional changes of murine embryonic stem cells, stromal cells and lung cancer cells

Project description:Transcriptional profiling of 3D and 2D condition on mouse embryonic stem cells, stromal cells (M210B4) and Lewis lung cancer cells (LLC).

2023-08-10 | GSE99942 | GEO

Expression data from the subclones of Lewis Lung Carcinoma (LLC) cells

Project description:Medium conditioned by LLC cells stimulates thermogenic gene expression when added onto primary adipocytes. We generated single cell colonies from parental LLC cells. Media conditioned by the subclones stimulated thermogenic gene expression in primary adipocytes at varying degrees. Subclones 2, 3, 6 and 28 produce significantly larger amount of thermogenic activity than the subclones 18, 19, 23 and 24. We compared gene expression profiles of these subclones to identify secreted factors enriched in the more thermogenic clones. LLC subclones were cultured under conditioned medium preparation conditiones and total RNA was extracted from biological replicates.

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Lewis lung carcinoma cells and TDAG8 overexpression

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Cancer-cell transcriptional signatures associated with Socs1 knockdown in an immunocompetent and orthotopic mouse model of non-small lung cancer

Project description:How cancer cells respond to signals coming from the tumor microenvironment cannot be fully elucidated in vitro. Additionally, RNA-Sequencing data from bulk human tumors does not assess the contribution of cancer cells alone, but from a complex mixture of both host and cancer cells. Using an immunocompetent and orthotopic mouse model of human Non-small Cell Lung Cancer, we sought to determine cancer cell specific transcriptomes from bulk tumors and compared these to cancer cells grown in vitro. We hypothesized that knockdown of Socs1, or suppressor of cytokine signaling 1 in Lewis Lung Carcinoma (LLC) cells would not only impact their response to single-agent immunotherapy, but also lead to complex changes in the tumor microenvironment. First, we harvested RNA from either LLC-NT (LLC cells transduced with a non-targeting control vector) or LLC-sh21 (LLC cells transduced with a Socs1 knockdown vector) cell lines grown in vitro. To determine the transcriptome of these cells grown in vivo, we injected LLC-NT or LLC-sh21 cells into the left lung of transgenic GFP-expressing C57BL/6J mice. After three weeks, tumor-bearing lung lobes were isolated from mice and were made into single cell suspensions. The GFP-negative cancer cell population was sorted from GFP-positive host cells by fluorescence activated cell sorting. RNA was extracted from freshly isolated cancer cells grown in vitro or from cancer cells isolated from tumors. Our RNA-seq analysis shows that LLC-sh21 cells respond to IFNg coming from the microenvironment relative to LLC-NT cells which have a blunted response due to high basal expression of SOCS1. These data suggest that specific genes in the IFNg response pathway are important for response to immunotherapy.

2019-05-23 | GSE131271 | GEO

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2016-08-10 | E-GEOD-76397 | biostudies-arrayexpress

Identification of acidic extracellular pH-modulated genes

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Gene expression signatures for primary tumor and tumor-derived exosomes

Project description:To determine the different gene signatures between primary tumor and tumor-derived exosomes, we have employed RNA-sequencing as a discovery platform to identify gene signatures of tumor-derived exosomes, taking the original tumors as a control. We subcutaneously inoculated C57BL/6 mice with Lewis lung carcinoma (LLC). Three weeks later, tumor tissues were cut and tumor-derived exosomes were isolated as described in the "treatment protocol". Then, both exosomal RNA and tumor RNA were extracted and sequenced. From sequencing, we found that exosomal RNAs showed quite different transcript profiles from tumor RNAs. Examination of different gene signatures between primary tumor and tumor-derived exosomes. 2 replicates each.

2016-08-10 | E-GEOD-80678 | biostudies-arrayexpress

Lewis lung carcinoma cells and TDAG8 overexpression

Project description:We observed the effects of TDAG8-overexpression in Lewis lung carcinoma (LLC) cells on the gene expression pattern. TDAG8-overexpressing or vector-transfected control LLC cells (5 × e5 in 200 µL of HBSS) were injected into the lateral tail veins of 6–8-week-old C57BL/6 mice. Lungs were removed on day 19 post-injection, and total RNA was extracted.

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