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Genome wide transcriptional response to altered levels of the Rpb7 subunit of RNA polymerase II identifies its role in DNA damage response in Schizosaccharomyces pombe

ABSTRACT: RNA Pol II is a highly conserved and multisubunit protein complex composed of 12 different subunits. Rpb4 and Rpb7 are small subunits and uniquely present in RNA Pol II in eukaryotes. Rpb7 is essential and highly conserved protein among all eukaryotes. However, we have a limited understanding of the functions of Rpb7 subunit. Therefore, in the present study, we have used whole genome microarrays to compare the transcriptional response of S. pombe cells expressing low levels of rpb7+ with wild type cells.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE119203 | GEO | 2019/02/27

REPOSITORIES: GEO

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Schizosaccharomyces pombe Pol II Transcription Elongation Factor ELL Functions as Part of a Rudimentary Super Elongation Complex

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Project description:Rpb4/7 binds RNA Polymerase II (Pol II) transcripts co-transcriptionally and accompanies them throughout their lives. By virtue of its capacity to interact with key regulators (e.g., Pol II, eIF3, Pat1) both temporarily and spatially, Rpb4/7 regulates the major stages of the mRNA lifecycle. Here we show that Rpb4/7 can undergo over 100 combinations of post-translational modifications (PTMs). Remarkably, the Rpb4/7 PTMs repertoire changes as the mRNA/Rpb4/7 complex progresses from one stage to the next. A mutagenesis approach in residues that undergo PTMs suggests that temporal Rpb4 PTMs regulate its interactions with key regulators of gene expression that control transcriptional and post-transcriptional stages. Moreover, one mutant type specifically affects mRNA synthesis despite its normal association with Pol II, whereas the other affects both mRNA synthesis and decay; both types disrupt the balance between mRNA synthesis and decay (‘mRNA buffering’) and the cell’s capacity to respond to the environment. Taken together, we propose that temporal Rpb4/7 PTMs are involved in cross talks among the various stages of the mRNA lifecycle.

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