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H3K4me3 ChIP-seq from B6CASTF1-PRDM9 Humanized/CAST testes

ABSTRACT: We report testis H3K4me3 enrichment in an F1 male from a C57BL/6J (B6) x CAST/Eij (CAST) cross (B6 mother, CAST father). This mouse is heterozygous at PRDM9 for a humanized allele (Davies et al. Nature 2016) and the CAST allele. After filtering of promoter H3K4me3 regions, these data serve as a measure of PRDM9 binding enrichment on each homologue. We found that both crossovers and non-crossovers (observed by sequencing F2/F4/F5 genomic DNA) are depleted at "asymmetric" Double-Strand Break hotspots where PRDM9 primarily binds only one of the two homologues. This proves that PRDM9 plays an important role in promoting inter-homologue interactions and can explain why increasing PRDM9 binding asymmetry predicts hybrid infertility. See Li, Bitoun, Altemose et al. 2018 (pending) for a complete summary.

ORGANISM(S): Mus musculus

PROVIDER: GSE119727 | GEO | 2018/09/28

REPOSITORIES: GEO

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Project description:Crossovers from B6xCAST F1 PRDM9-Humanized/CAST testes

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