Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HeLa cell response to overexpression of wild type and mutated forms of the P19 splice variant of c-H-ras

ABSTRACT: The c-H-ras proto-oncogene undergoes alternative splicing of the exon termed IDX giving rise to a novel protein of the Ras family, p19 (H-RasIDX). The experiment tests the effect on the transcriptome of overexpressing the wild type and W164A mutated forms of p19. Keywords: genetic modification

ORGANISM(S): Homo sapiens

PROVIDER: GSE12059 | GEO | 2009/12/30

SECONDARY ACCESSION(S): PRJNA113391

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

HeLa cell response to overexpression of wild type and mutated forms of the P19 splice variant of c-H-ras

Project description:The c-H-ras proto-oncogene undergoes alternative splicing of the exon termed IDX giving rise to a novel protein of the Ras family, p19 (H-RasIDX). The experiment tests the effect on the transcriptome of overexpressing the wild type and W164A mutated forms of p19. Keywords: genetic modification Three-condition experiment, where wild type p19 H-ras transfection or transfection with a mutated form (p19W164A) were compared to transfection with the negative control (empty pRK5 expression vector) which was used as the common reference. Biological replicates: 3 of each set of treatments, independently grown and harvested. Two technical replicates with dye swapping per comparison.

2009-12-29 | E-GEOD-12059 | biostudies-arrayexpress

MicroRNA profiling of human HeLa cells overexpressing p19 H-Ras

Project description:MicroRNA miRNA expression profiles for human HeLa cells (Cervical cancer), overexpressing p19 H-Ras, were examined to investigate the miRNA regulation by p19 H-Ras. miRNA microarray analysis identified statistical unique profiles, which could discriminate miRNAs regulated by p19 H-Ras and not regulated by the p19 mutant (W164A) H-Ras.

2008-12-12 | E-TABM-494 | biostudies-arrayexpress

iCLIP mapping of coilin-RNA interactions in HeLa and P19 cells

Project description:Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells expressing GFP fused to a nuclear localization signal (GFP-NLS) was used as a negative control. iCLIP results revealed that coilin binds several classes of ncRNA including snRNAs, U3 snoRNA and scaRNAs. Interestlignly the majority of coilin targets were intronic snoRNAs, suggesting a novel role of CBs in snoRNA biogenesis.

2014-11-06 | GSE58448 | GEO

p19-WT and p19-T111BpyAla treatment of extracted small RNAs

Project description:small RNA fractions were treated with either p19-WT or T111BpyAla to evaluate the catalytic ability of p19-T111BpyAla compared to the WT protein in degrading miRNAs

2023-04-28 | GSE229272 | GEO

iCLIP mapping of coilin-RNA interactions in HeLa and P19 cells

2014-11-06 | E-GEOD-58448 | biostudies-arrayexpress

Gene expression profiling in Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid

2010-07-10 | GSE21724 | GEO

Gene expression profiling in Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid

Project description:Analysis of senataxin effect on neuronal differentiation and neurite growth in RA-treated P19 cells by modulating senataxin expression levels. Senataxin silencing does not grossly affect the gene expression profiles of P19 cells At day one total RNA from Setx shRNA P19 cells, wild type human Setx P19 transfected cells with and without retionoic acid were used to compare gene expression profiling versus P19 control cells

2010-07-10 | E-GEOD-21724 | biostudies-arrayexpress

Peptide mapping of SRSF7_RRM isoforms in murine P19 cells

Project description:Here we mapped peptides of mouse SRSF7_RRM purified from P19 cells.

2020-05-27 | PXD016884 | Pride

P19 embryonic carcinoma (EC) cells induced to form cardiomyocytes in vitro

Project description:SAGE identification of differentiation responsive genes in P19 embryonic cells induced to form cardiomyocytes in vitro. P19 embryonic carcinoma (EC) cells, induced to form cardiomyocytes in vitro - undifferentiated cells, day 3+0.5 and day 3+3.0 of differentiation protocol. Keywords = EC cells, P19, differentiation, cardiomyocytes Keywords: time-course

2002-07-18 | GSE42 | GEO

p19-WT and p19-T111BpyAla treatment of extracted small RNAs

Project description:p19-WT and p19-T111BpyAla treatment of extracted small RNAs

| PRJNA953919 | ENA