ICLIP mapping of coilin-RNA interactions in HeLa and P19 cells
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ABSTRACT: Our ChIP resuls suggested that coilin association with U3, snRNA and histone genes might be dependent on coilin-RNA interaction. We used iCLIP of coilin-GFP expressed in HeLa and P19 cell lines at endogenous levels to identify coilin RNA targets and investigate RNA-binding specificity. P19 cells expressing GFP fused to a nuclear localization signal (GFP-NLS) was used as a negative control. iCLIP results revealed that coilin binds several classes of ncRNA including snRNAs, U3 snoRNA and scaRNAs. Interestlignly the majority of coilin targets were intronic snoRNAs, suggesting a novel role of CBs in snoRNA biogenesis.
ORGANISM(S): Mus musculus Homo sapiens
PROVIDER: GSE58448 | GEO | 2014/11/06
SECONDARY ACCESSION(S): PRJNA252666
REPOSITORIES: GEO
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