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A technique to detect native R-loops genome-wide without immunoprecipitation

ABSTRACT: We adapted the nuclease-targeting approach CUT&RUN (Skene & Henikoff, eLife 2017) to map R-loops in vivo. We show that this approach accurately identifies R-loops that are sensitive to both Actinomycin D and RNase H and largely overlap with previously identified regions containing R-loops. We also show that MapR can identify R-loops genome-wide from much smaller input material than existing techniques.

ORGANISM(S): Homo sapiens

PROVIDER: GSE120637 | GEO | 2019/10/18

REPOSITORIES: GEO

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A technique to detect native R-loops genome-wide without immunoprecipitation

Project description:A technique to detect native R-loops genome-wide without immunoprecipitation

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