Transcriptomics

Dataset Information

0

Hippo kinases Mst1 and Mst2 sense and amplify IL-2R−STAT5 signaling in Treg cells to establish stable regulatory activity


ABSTRACT: Interleukin-2 (IL-2) and downstream transcription factor STAT5 are important for maintaining regulatory T (Treg) cell homeostasis and function. Tregs can respond to low levels of IL-2, but the mechanisms by which STAT5 is activated during partial IL-2 deficiency remain uncertain. We identified the serine-threonine kinase Mst1 as a signal-dependent amplifier of IL-2−STAT5 activity in Tregs. Tregs had high Mst1 and Mst2 (Mst1−Mst2) activity that was crucial to prevent tumor resistance and autoimmunity. Mechanistically, Mst1−Mst2 sensed IL-2 signals to promote the STAT5 activation necessary for Treg cell homeostasis and lineage stability, and maintain the highly suppressive phophorylated-STAT5+ Treg cell subpopulation. Unbiased quantitative proteomics revealed an association of Mst1 with the cytoskeletal DOCK8−LRCHs module that shaped activity Rho-family GTPase Rac activity, which in turn mediated STAT5 activation in Tregs. Collectively, IL-2−STAT5 signaling critically depends upon Mst1−Mst2 functions to maintain a stable Treg cell pool and immune tolerance. We used microarrays to compare the global transcription profiles of WT and Mst1/Mst2-null Treg cell populations

ORGANISM(S): Mus musculus

PROVIDER: GSE120711 | GEO | 2018/11/08

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA494226 | ENA
2022-02-16 | GSE162030 | GEO
2022-02-16 | GSE163946 | GEO
2020-04-18 | GSE144776 | GEO
2022-09-14 | GSE185401 | GEO
2014-01-02 | E-GEOD-50219 | biostudies-arrayexpress
2021-05-11 | GSE164937 | GEO
2015-01-01 | E-GEOD-61582 | biostudies-arrayexpress
2015-01-01 | E-GEOD-61627 | biostudies-arrayexpress
2014-01-02 | GSE50219 | GEO